EC HPLC column (analytical), NUCLEODUR 100-5 NH2-RP, 5 µm, 125x2 mm

EC HPLC column (analytical), NUCLEODUR 100-5 NH2-RP, 5 µm, 125x2 mm
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760730.20 02402027006505
Package unit 1 Piece(s)
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This HPLC column is packed with polar aminopropyl modification, based on high purity NUCLEODUR... more

This HPLC column is packed with polar aminopropyl modification, based on high purity NUCLEODUR silica gel. This HPLC phase is able to generate a clearly recognizable different retention behavior compared to purely alkyl-functionalized surface modifications. Besides cyano modifications, amino modifications belong to the most frequently used polar silica phases. Amino phases can be run in the RP mode using aqueous-organic eluent mixtures as well as in the NP mode, e.g., with hexane as mobile phase. Also anion exchange chromatography of anions and organic acids using common buffers (e.g., acetate or phosphate) in conjunction with organic modifiers (e.g., acetonitrile) is possible. Separation mechanism based on polar interactions, hydrophobic interactions and weak ion exchange interactions.

Hazardous material No
Method HPLC, analytical
Brand NUCLEODUR
Base material NUCLEODUR - high purity silica
Phase NUCLEODUR 100-5 NH2-RP
USP listing L8
Surface chemistry Aminopropyl (NH2) modification
Mode Multi-mode (RP and NP), Reversed phase (RP)
Type EC HPLC column (analytical)
Hardware Stainless steel
Column length 125 mm
Column inner diameter 2 mm
Particle type Fully porous particles (FPP)
Particle shape Spherical
Particle size 5 µm
Eluent in HPLC column Acetonitrile – water
Recommended application(s) HPLC, Hydrocarbons under NP conditions, Polar compounds under RP conditions (sugars, DNA bases)
Endcapped No
Carbon content 2.5 %
pH stability 2.0–8.0
Pore size 110 Å
Specific surface according to BET 340 m²/g
Pore volume 0.9 mL/g
Temperature stability (max) 60 °C
Tmax (phosphate buffer) 40 °C
Pressure stability (max) 600 bar
Column volume 0.37 mL
Storage temperature RT
Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis