VP HPLC column (preparative), NUCLEODUR PolarTec, 5 µm, 250x10 mm
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Based on high-purity NUCLEODUR silica gel, a polar group was embedded in a hydrophobic C18 modification. As a result, this HPLC phase shows steric and an increased polar selectivity. This HPLC phase shows a good performance under high aqueous conditions. It is a hydrophobic phase with pronounced hydrophilic properties. Separation mechanism based on hydrophobic (van der Waals) and polar interactions. Suitable for LC/MS due to low bleeding characteristics.
| Base material | NUCLEODUR - high purity silica |
|---|---|
| Brand | NUCLEODUR |
| Carbon content | 17.0 % |
| Column inner diameter | 10 mm |
| Column length | 250 mm |
| Column volume | 20 mL |
| Eluent in HPLC column | Acetonitrile – water |
| Endcapped | Yes |
| Gross weight (incl. packaging) | 494.07 g / 1.09 lbs |
| Hardware | Stainless steel |
| Hazardous material | No |
| Method | HPLC, preparative |
| Mode | Reversed phase (RP) |
| Packaging dimensions | 400 x 115 x 54 mm / 15.75 x 4.53 x 2.13 Inch |
| Particle shape | Spherical |
| Particle size | 5 µm |
| Particle type | Fully porous particles (FPP) |
| pH stability | 1.0–9.0 |
| Phase | NUCLEODUR PolarTec |
| Pore size | 110 Å |
| Pressure stability (max) | 400 bar / 5800 psi |
| Recommended application(s) | For examples please see analytical NUCLEODUR PolarTec, RP applications (preparative) |
| Recommended flow rate | 3–9 mL/min |
| Scope of delivery | 1x preparative HPLC column, instruction leaflet, certificate of analysis |
| Specific surface according to BET | 340 m²/g |
| Storage temperature | 15–25 °C / 59–77 °F |
| Surface chemistry | Octadecyl phase (C18, ODS) modification, with embedded polar group (PEG) |
| Temperature stability (max) | 60 °C |
| Type | VP HPLC column (preparative) |
| USP listing | L1, L60 |