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VP HPLC column (preparative), NUCLEODUR PFP, 5 µm, 250x21 mm

This hydrophobic phase with pentafluorophenyl-propyl modification is based on high purity NUCLEODUR silica gel and gives an alternative selectivity in comparison to classical C18 modifications. Separation mechanism based on four retention mechanisms - polar interactions (H bonds), dipole-dipole interactions, π-π interactions and hydrophobic interactions. Suitable for LC/MS due to low bleeding characteristics.

Base material NUCLEODUR - high purity silica
Brand NUCLEODUR
Carbon content 8.0 %
Column inner diameter 21 mm
Column length 250 mm
Column volume 87 mL
Eluent in HPLC column Acetonitrile – water
Endcapped Yes
Gross weight (incl. packaging) 1366.3 g / 3.01 lbs
Hardware Stainless steel
Hazardous material No
Method HPLC, preparative
Mode Reversed phase (RP)
Packaging dimensions 400 x 115 x 54 mm / 15.75 x 4.53 x 2.13 Inch
Particle shape Spherical
Particle size 5 µm
Particle type Fully porous particles (FPP)
pH stability 1.0–9.0
Phase NUCLEODUR PFP
Pore size 110 Å
Pressure stability (max) 400 bar / 5800 psi
Recommended application(s) For examples please see analytical NUCLEODUR PolarTec, RP applications (preparative)
Recommended flow rate 14–40 mL/min
Scope of delivery 1x preparative HPLC column, instruction leaflet, certificate of analysis
Specific surface according to BET 340 m²/g
Storage temperature 15–25 °C / 59–77 °F
Surface chemistry Pentafluorophenylpropyl (PFP) modification
Temperature stability (max) 60 °C
Type VP HPLC column (preparative)
USP listing L43