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NucleoType DirectLyse Reagent, lysis reagent for direct PCR / qPCR applications

The NucleoType DirectLyse Reagent serves an universal lysis buffer designed for simple DNA extraction from diverse biological samples, such as animal tissue, hair samples, cultivated cells, blood, swabs, or plant tissue. The reagent is intended for simple DNA extraction, enabling direct use in subsequent PCR or qPCR reactions. Comprising Extraction Buffer XLR and Liquid Proteinase K, this single-tube and column-free DNA extraction reagent can process up to 500 samples, depending on the sample type and protocol employed.

Application Direct PCR or direct qPCR for genotyping, transgenic identification, identity testing, multiplex PCR, meld point analysis, or pathogen DNA detection
Brand NucleoType
CE certified No, research use only
Format Column-free lysis reagent for manual use
Gross weight (incl. packaging) 100.85 g / 0.22 lbs
Hazardous material Yes
Packaging dimensions 53 x 53 x 104 mm / 2.09 x 2.09 x 4.09 Inch
Preparation time 10-15 min
Sample amount Few cells up to approx. 200 mg biological sample material (tissue, blood, hair, plant tissue, plant seeds, swabs,...)
Sample material Animal tissue, Blood, Blood spots, Blood storage card, Cells, Ear punches, Food, Hair samples, Human tissue, Mouse samples, Plant, Seed, Swabs
Scope of delivery Extraction Buffer XLR (60 mL) and Liquid Proteinase K (1250 µL)
Shelf life (from production) 24 Month(s)
Storage temperature 15–25 °C / 59–77 °F
Target DNA
Technology Lysis reagent for direct PCR / qPCR

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