NucleoSpin Tissue, Mini kit for DNA from cells and tissue
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NucleoSpin Tissue is a spin column based kit for manual use. It is intended for the isolation of genomic DNA from a large variety of sample types. Besides spin columns and buffers it contains Proteinase K for sample digestion. It is an allrounder kit with more than a dozen support protocols.
| Application | Isolation of DNA |
|---|---|
| Automated use | No |
| Brand | NucleoSpin |
| CE certified | No, research use only |
| Elution volume | 60–100 µL |
| Format | Mini prep |
| Fragment size | 200 bp–approx. 50 kbp |
| Handling | Centrifugation |
| Hazardous material | Yes |
| Lysate clarification | Centrifugation |
| Preparation time | 20 min/prep (excl. lysis) |
| Sample amount | < 25 mg human / animal tissue, 10²–10⁷ cultured cells |
| Sample material | Animal tissue, Cells, Human tissue |
| Selling unit | 10 Prep(s), 50 Prep(s), 250 Prep(s) |
| Shelf life (from production) | 27 Month(s) |
| Storage temperature | 15–25 °C / 59–77 °F |
| Target | DNA |
| Technology | Silica membrane technology |
| Theoretical binding capacity | 60 µg |
| Typical downstream application | Multiplex PCR, Next Generation Sequencing, enzymatic reactions |
| Typical purity A260/A280 | 1.7–1.9 |
| Typical yield | 20–35 µg |