NucleoSpin RNA, Mini kit for RNA purification
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NucleoSpin RNA is a spin column based kit for manual use. It is intended for the isolation of high purity and integrity RNA from a wide range of sample materials like tissues, cultured cells, yeast and bacteria. Besides spin columns and buffers it contains rDNAse for on column DNA digestion.
| Application | Isolation of RNA |
|---|---|
| Automated use | No |
| Brand | NucleoSpin |
| CE certified | No, research use only |
| Elution volume | 30–120 µL |
| Format | Mini prep |
| Fragment size | > 200 nt |
| Handling | Centrifugation, vacuum |
| Hazardous material | Yes |
| Lysate clarification | Centrifugation, NucleoSpin Filter |
| Preparation time | 35 min/6 preps |
| Sample amount | < 5 x 10⁶ cultured cells, < 30 mg human / animal tissue, < 10⁹ bacterial cells, < 10⁸ yeast cells |
| Sample material | Animal tissue, Bacteria, Cells, Human tissue, Yeast |
| Selling unit | 4 Prep(s), 10 Prep(s), 50 Prep(s), 250 Prep(s) |
| Shelf life (from production) | 24 Month(s) |
| Storage temperature | 15–25 °C / 59–77 °F |
| Target | RNA |
| Technology | Silica membrane technology |
| Theoretical binding capacity | 200 µg |
| Typical downstream application | RNA-Seq, RT−PCR |
| Typical purity A260/A280 | 1.9–2.1 |
| Typical RIN | > 9 |
| Typical yield | 14 µg (10⁶ HeLa cells), 70 µg (10⁹ bacterial cells) |