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NucleoSpin Plasmid Transfection-grade Plus, Mini kit for ultrapure plasmid DNA

NucleoSpin Plasmid Transfection-grade Plus is the new generation for silica membrane-based plasmid isolation. This kit allows the isolation of up to 100 µg of ultrapure plasmid DNA with endotoxin levels ≤ 1 EU/µg plasmid DNA.

Application Transfection-grade plasmid DNA isolation
Automated use No
Brand NucleoSpin
CE certified No, research use only
Elution volume 30−50 µL
Endotoxin level ≤ 1 EU/μg DNA
Format Mini prep
Handling Centrifugation, Vacuum
Hazardous material Yes
Lysate clarification Centrifugation
Preparation time 20 min/6 preps
Sample amount 1−5 mL
Sample material Bacteria, E. coli
Selling unit 10 Prep(s), 50 Prep(s), 250 Prep(s)
Shelf life (from production) 24 Month(s)
Storage temperature 15–25 °C / 59–77 °F
Target Plasmid DNA
Technology Silica membrane technology and endotoxin reduction technology
Theoretical binding capacity 100 µg
Typical downstream application Transfection of sensitive cells and cell injections
Typical purity A260/A280 1.8−1.85
Typical yield 35-60 µg
Vector size < 25 kbp