Skip to main content Skip to search Skip to main navigation

NucleoBond Xtra Midi Columns for transfection-grade plasmid DNA

Seperate columns for all our NucleoBond Xtra Midi kits.

Application Transfection-grade plasmid DNA isolation
Automated use Yes, can be automated on the Andrew+ pipetting robot
Brand NucleoBond
CE certified No, research use only
Endotoxin level 1−10 EU/µg DNA
Format Midi prep
Gross weight (incl. packaging) 21080 g / 46.47 lbs
Handling Gravity flow
Hazardous material No
Isopropanol precipitation Centrifugation, NucleoBond Finalizer, NucleoSnap Finisher Midi, NucleoSpin Finisher Midi
Lysate clarification Column filter
Packaging dimensions 800 x 600 x 620 mm / 31.50 x 23.62 x 24.41 Inch
Preparation time 70 min/prep
Sample amount < 200 mL (high copy), < 400 mL (low copy)
Sample material Bacteria, E. coli
Shelf life (from production) 72 Month(s)
Storage temperature 15–25 °C / 59–77 °F
Target Plasmid DNA
Technology Anion exchange chromatography
Theoretical binding capacity 1000 µg
Typical downstream application Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells
Typical purity A260/A280 1.8−1.95
Typical yield 500 µg
Vector size < 300 kbp