EC UHPLC column (analytical), NUCLEODUR HILIC, 1.8 µm, 50x2 mm
Separation science is always looking for new and effective strategies to accomplish the tasks of modern analytics. Especially for polar compounds reversed phase HPLC – the most common analytical method – is often limited. Here, hydrophilic stationary phases provide an additional tool for the separation of polar analytes in HPLC. HILIC is NP chromatography of polar and ionic compounds under RP conditions. The zwitterionic HILIC phase is based on high purity NUCLEODUR silica gel.
| Base material | NUCLEODUR - high purity silica |
|---|---|
| Brand | NUCLEODUR |
| Carbon content | 7.0 % |
| Column inner diameter | 2 mm |
| Column length | 50 mm |
| Column volume | 0.15 mL |
| Eluent in HPLC column | Acetonitrile – water |
| Endcapped | No |
| Gross weight (incl. packaging) | 168 g / 0.37 lbs |
| Hardware | Stainless steel |
| Hazardous material | No |
| Method | HPLC/UHPLC, analytical |
| Mode | Hydrophilic Interaction Chromatography (HILIC) |
| Packaging dimensions | 235 x 117 x 54 mm / 9.25 x 4.61 x 2.13 Inch |
| Particle shape | Spherical |
| Particle size | 1.8 µm |
| Particle type | Fully porous particles (FPP) |
| pH stability | 2.0–8.5 |
| Phase | NUCLEODUR HILIC |
| Pore size | 110 Å |
| Pressure stability (max) | 900 bar / 13050 psi |
| Recommended application(s) | Amino acids, HILIC applications UHPLC, Highly polar analytes, Hydrophilic compounds, Nucleosides, Oligonucleotides, Organic polar acids and bases, Peptides, Polar natural compounds, Water-soluble vitamins |
| Scope of delivery | 1x analytical HPLC/UHPLC column, instruction leaflet, certificate of analysis |
| Specific surface according to BET | 340 m²/g |
| Storage temperature | 15–25 °C / 59–77 °F |
| Surface chemistry | Ammonium – sulfonic acid (zwitterionic) modification |
| Temperature stability (max) | 60 °C |
| Type | EC UHPLC column (analytical) |
| USP listing | Not available or proprietary |
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