EC HPLC column (analytical), NUCLEOSIL 300-5 C4 MPN, 5 µm, 250x4 mm
Special reversed C4 (butyl) phase with monomerically bonded alkyl chains, brush type structure. Based on the original NUCLEOSIL silica gel. It is one of the first spherical silicas for HPLC and was originally developed by MACHEREY-NAGEL in 1974. It is exclusively manufactured in Germany and is still one of the most used HPLC silicas in quality control around the globe. Predominantly hydrophobic forces with a small portion of hydrophilic interactions. The dynamic protein binding capacity per g packing - 14 mg BSA, 27 mg cytochrome C. especially suited for the purification of larger, hydrophobic peptides and very different proteins. Maximum separation efficiency can be achieved when the injected protein mass does not exceed 1–2 % of the maximum protein loading capacity.
Are you interested in real-world applications for this product? Click here to view the application notes.
| Base material | NUCLEOSIL - the standard silica |
|---|---|
| Brand | NUCLEOSIL |
| Carbon content | Not available or proprietary |
| Column inner diameter | 4 mm |
| Column length | 250 mm |
| Eluent in HPLC column | Methanol |
| Endcapped | No |
| Gross weight (incl. packaging) | 238.77 g / 0.52 lbs |
| Hardware | Stainless steel |
| Hazardous material | No |
| Method | HPLC, analytical |
| Mode | Special application or diverse |
| Packaging dimensions | 400 x 115 x 54 mm / 15.75 x 4.53 x 2.13 Inch |
| Particle shape | Spherical |
| Particle size | 5 µm |
| Particle type | Fully porous particles (FPP) |
| pH stability | 1.0–9.0 |
| Phase | NUCLEOSIL C4 MPN 300-5 |
| Pore size | 300 Å |
| Pressure stability (max) | 250 bar / 3625 psi |
| Recommended application(s) | Biological macromolecules, HPLC |
| Scope of delivery | 1x analytical HPLC column, instruction leaflet, certificate of analysis |
| Specific surface according to BET | 100 m²/g |
| Storage temperature | 15–25 °C / 59–77 °F |
| Surface chemistry | Special butyl (C4) modification |
| Temperature stability (max) | 60 °C |
| Type | EC HPLC column (analytical) |
| USP listing | L26 |
Accessories
Content 1 Set(s)