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EC HPLC column (analytical), NUCLEOSIL 300-5 C4, 5 µm, 250x4.6 mm

This HPLC column is packed with butyl modification, based on the original NUCLEOSIL silica gel. It is one of the first spherical silicas for HPLC and was originally developed by MACHEREY-NAGEL in 1974. It is exclusively manufactured exclusively in Germany and is still one of the most used HPLC silicas in quality control around the globe.

Base material NUCLEOSIL - the standard silica
Brand NUCLEOSIL
Carbon content 2.0 %
Column inner diameter 4.6 mm
Column length 250 mm
Column volume 4.15 mL
Eluent in HPLC column Acetonitrile – water
Endcapped Yes
Gross weight (incl. packaging) 231.67 g / 0.51 lbs
Hardware Stainless steel
Hazardous material No
Method HPLC, analytical
Mode Reversed phase (RP)
Packaging dimensions 400 x 115 x 54 mm / 15.75 x 4.53 x 2.13 Inch
Particle shape Spherical
Particle size 5 µm
Particle type Fully porous particles (FPP)
pH stability 2.0–8.0
Phase NUCLEOSIL C4 300-5
Pore size 300 Å
Pressure stability (max) 300 bar / 4350 psi
Recommended application(s) Ion-pairing chromatography, RP applications HPLC, Reversed phase
Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis
Specific surface according to BET 100 m²/g
Storage temperature 15–25 °C / 59–77 °F
Surface chemistry Medium density monomeric butyl (C4) wide-pore modification
Temperature stability (max) 60 °C
Type EC HPLC column (analytical)
USP listing L26

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