EC HPLC column (analytical), NUCLEOSIL 100-5 CN, 5 µm, 250x4.6 mm
The cyanopropyl phase is able to generate a clearly recognizable different retention behavior compared to purely alkyl-functionalized surface modifications. The polarity can be classified as intermediate based on multiple retention mechanisms such as dipole-dipole, π-π, and also hydrophobic interactions. Therefore, CN phases show a distinct selectivity for polar organic compounds as well as for molecules containing π-electron systems (e.g., analytes with double bonds). This HPLC phase can be run in the RP mode using aqueous-organic eluent mixtures as well as in the NP mode, e.g., with hexane as mobile phase. This HPLC column is packed with CN modification, based on the original NUCLEOSIL silica gel. It is one of the first spherical silicas for HPLC and was originally developed by MACHEREY-NAGEL in 1974. It is exclusively manufactured in Germany and is still one of the most used HPLC silicas in quality control around the globe.
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| Base material | NUCLEOSIL - the standard silica |
|---|---|
| Brand | NUCLEOSIL |
| Carbon content | 5.0 % |
| Column inner diameter | 4.6 mm |
| Column length | 250 mm |
| Column volume | 4.15 mL |
| Eluent in HPLC column | n-heptane |
| Endcapped | No |
| Gross weight (incl. packaging) | 231.67 g / 0.51 lbs |
| Hardware | Stainless steel |
| Hazardous material | No |
| Method | HPLC, analytical |
| Mode | Multi-mode (NP and RP), Normal phase (NP) |
| Packaging dimensions | 400 x 115 x 54 mm / 15.75 x 4.53 x 2.13 Inch |
| Particle shape | Spherical |
| Particle size | 5 µm |
| Particle type | Fully porous particles (FPP) |
| pH stability | 2.0–8.0 |
| Phase | NUCLEOSIL CN 100-5 |
| Pore size | 100 Å |
| Pressure stability (max) | 400 bar / 5800 psi |
| Recommended application(s) | HPLC, Organic acids, Reversed phase and normal phase chromatography, Steroids, Tricyclic antidepressants |
| Scope of delivery | 1x analytical HPLC column, instruction leaflet, certificate of analysis |
| Specific surface according to BET | 350 m²/g |
| Storage temperature | 15–25 °C / 59–77 °F |
| Surface chemistry | Cyano, nitrile (CN) modification |
| Temperature stability (max) | 60 °C |
| Type | EC HPLC column (analytical) |
| USP listing | L10 |
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