EC HPLC column (analytical), NUCLEOSIL 100-5 C18 PPN, 5 µm, 250x4 mm
US$1,045.00*
*taxes and shipping not included
Special reversed phase with polymerically bonded alkyl chains with exclusively hydrophobic interactions. Based on the original NUCLEOSIL silica gel. It is one of the first spherical silicas for HPLC and was originally developed by MACHEREY-NAGEL in 1974. It is exclusively manufactured in Germany and is still one of the most used HPLC silicas in quality control around the globe. Dynamic protein binding capacity per g packing - 8 mg BSA, 64 mg cytochrome C. Suited for the separation of peptides and proteins up to about 40 kD, also suited for basic peptides.
| Base material | NUCLEOSIL - the standard silica |
|---|---|
| Brand | NUCLEOSIL |
| Carbon content | Not available or proprietary |
| Column inner diameter | 4 mm |
| Column length | 250 mm |
| Eluent in HPLC column | Methanol |
| Endcapped | No |
| Gross weight (incl. packaging) | 238.77 g / 0.52 lbs |
| Hardware | Stainless steel |
| Hazardous material | No |
| Method | HPLC, analytical |
| Mode | Special application or diverse |
| Packaging dimensions | 400 x 115 x 54 mm / 15.75 x 4.53 x 2.13 Inch |
| Particle shape | Spherical |
| Particle size | 5 µm |
| Particle type | Fully porous particles (FPP) |
| pH stability | 1.0–9.0 |
| Phase | NUCLEOSIL C18 PPN 100-5 |
| Pore size | 100 Å |
| Pressure stability (max) | 250 bar / 3625 psi |
| Recommended application(s) | Biological macromolecules, HPLC, Large peptides, Medium-size hydrophilic proteins |
| Scope of delivery | 1x analytical HPLC column, instruction leaflet, certificate of analysis |
| Specific surface according to BET | 350 m²/g |
| Storage temperature | 15–25 °C / 59–77 °F |
| Surface chemistry | Special octadecyl (C18, ODS) modification |
| Temperature stability (max) | 60 °C |
| Type | EC HPLC column (analytical) |
| USP listing | L1 |