EC HPLC column (analytical), NUCLEODUR 300-5 C4 ec, 5 µm, 250x3 mm
Wide pore RP phase for daily routine analysis. The medium density butyl modification is based on high purity NUCLEODUR silica gel and is often used for the separation of biomolecules. Butyl (C4) modifications have a lower retention for hydrophobic compounds compared to C18 (octadecyl silane, ODS) or C8 (octyl, MOS) modifications.
| Base material | NUCLEODUR - high purity silica |
|---|---|
| Brand | NUCLEODUR |
| Carbon content | 2.5 % |
| Column inner diameter | 3 mm |
| Column length | 250 mm |
| Column volume | 1.75 mL |
| Eluent in HPLC column | Acetonitrile – water |
| Endcapped | Yes |
| Gross weight (incl. packaging) | 304 g / 0.67 lbs |
| Hardware | Stainless steel |
| Hazardous material | No |
| Method | HPLC, analytical |
| Mode | Reversed phase (RP) |
| Packaging dimensions | 400 x 115 x 54 mm / 15.75 x 4.53 x 2.13 Inch |
| Particle shape | Spherical |
| Particle size | 5 µm |
| Particle type | Fully porous particles (FPP) |
| pH stability | 1.0–9.0 |
| Phase | NUCLEODUR C4 ec 300-5 |
| Pore size | 300 Å |
| Pressure stability (max) | 450 bar / 6525 psi |
| Recommended application(s) | Biological macromolecules, HPLC, Peptides, Proteins |
| Scope of delivery | 1x analytical HPLC column, instruction leaflet, certificate of analysis |
| Specific surface according to BET | 100 m²/g |
| Storage temperature | 15–25 °C / 59–77 °F |
| Surface chemistry | Medium density monomeric butyl (C4) wide-pore modification |
| Temperature stability (max) | 60 °C |
| Type | EC HPLC column (analytical) |
| USP listing | L26 |
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