EC HPLC column (analytical), NUCLEODUR 100-3 NH2-RP, 3 µm, 125x2 mm
US$745.00*
*taxes and shipping not included
This HPLC column is packed with polar aminopropyl modification, based on high purity NUCLEODUR silica gel. This HPLC phase is able to generate a clearly recognizable different retention behavior compared to purely alkyl-functionalized surface modifications. Besides cyano modifications, amino modifications belong to the most frequently used polar silica phases. Amino phases can be run in the RP mode using aqueous-organic eluent mixtures as well as in the NP mode, e.g., with hexane as mobile phase. Also anion exchange chromatography of anions and organic acids using common buffers (e.g., acetate or phosphate) in conjunction with organic modifiers (e.g., acetonitrile) is possible. Separation mechanism based on polar interactions, hydrophobic interactions and weak ion exchange interactions.
| Base material | NUCLEODUR - high purity silica |
|---|---|
| Brand | NUCLEODUR |
| Carbon content | 2.5 % |
| Column inner diameter | 2 mm |
| Column length | 125 mm |
| Column volume | 0.37 mL |
| Eluent in HPLC column | Acetonitrile – water |
| Endcapped | No |
| Gross weight (incl. packaging) | 154.93 g / 0.34 lbs |
| Hardware | Stainless steel |
| Hazardous material | No |
| Method | HPLC, analytical |
| Mode | Multi-mode (NP and RP), Reversed phase (RP) |
| Packaging dimensions | 235 x 117 x 54 mm / 9.25 x 4.61 x 2.13 Inch |
| Particle shape | Spherical |
| Particle size | 3 µm |
| Particle type | Fully porous particles (FPP) |
| pH stability | 2.0–8.0 |
| Phase | NUCLEODUR NH2-RP 100-3 |
| Pore size | 110 Å |
| Pressure stability (max) | 600 bar / 8700 psi |
| Recommended application(s) | HPLC, Hydrocarbons under NP conditions, Polar compounds under RP conditions (sugars, DNA bases) |
| Scope of delivery | 1x analytical HPLC column, instruction leaflet, certificate of analysis |
| Specific surface according to BET | 340 m²/g |
| Storage temperature | 15–25 °C / 59–77 °F |
| Surface chemistry | Aminopropyl (NH2) modification |
| Temperature stability (max) | 60 °C |
| Type | EC HPLC column (analytical) |
| USP listing | L8 |
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