NucleoSpin DNA Yeast, Mini-Kit für die Isolierung genomischer DNA aus Hefen
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Isolation of total DNA from yeast and fungi – MN Bead Tubes Type C included
| Anwendung | Isolierung von DNA |
|---|---|
| Automatische Nutzung | Nein |
| CE-zertifiziert | Nein, nur für wissenschaftlichen Gebrauch |
| Elutionsvolumen | 100−200 µL |
| Format | Mini Präp |
| Fragmentgröße | 200 bp–ca. 50 kbp |
| Gefahrstoffe | Ja |
| Haltbarkeit (ab Produktion) | 24 Monat(e) |
| Handhabung | Zentrifugation |
| Lagertemperatur | 15–25 °C |
| Präparationszeit | 35 min/Präp |
| Probenmaterial | Hefe, Pilze |
| Probenmenge | < 100 mg Feuchtgewicht |
| Technologie | Silica-Membrantechnologie |
| Theoretische Bindungskapazität | 60 µg |
| Typische Ausbeute | Bis zu 20 µg (je nach Probenmenge und Qualität) |
| Typische Downstream-Anwendung | Echtzeit-PCR, PCR, enzymatische Reaktionen, qRT-PCR |
| Typische Reinheit A260/A280 | 1,6–2,0 |
| Verkaufseinheit | 10 Präp(s), 50 Präp(s) |
| Warenzeichen | NucleoSpin |
| Ziel | DNA |
NucleoSpin DNA Yeast
- Optimized lysis with MN Bead Tubes Type C
- Up to 100 mg of sample input
- High genomic DNA yields
For DNA from yeast: Don’t lyse hard, lyse smart!
Protocols for isolation of DNA from yeast typically include either a chemical treatment to weaken the cell walls or a mechanical lysis with glass beads. The former requires additional hands-on time, the latter often is inefficient leading to prolonged lysis times and low DNA yields. The NucleoSpin DNA Yeast kit contains MN Bead Tubes Type C with corundum beads for most efficient lysis. Using corundum beads improves the lysis resulting in a drastically increased DNA yield. The kit delivers superior yields for various, popular yeast species, including Saccharomyces cerevisiae, Schizosachharomyces pombe and Pichia pastoris.
NucleoSpin DNA Yeast product page MN Bead Tubes Type C product page
High DNA yields and efficient sample disruption from up to 100 mg sample
DNA was purified from 40–100 mg of cultured yeast (wet weight). DNA yield linearly increases with sample input.
Yeast samples (40 mg) were subjected to mechanical lysis with different types of beads. MN Bead Tubes Type C deliver the highest DNA yield in subsequent purification with NucleoSpin DNA Yeast kit.
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