NucleoSpin Finisher Midi zur Entsalzung und Konzentrierung von Plasmid DNA
Preis auf Anfrage
*ohne Steuern und Versandkosten
Fast concentration and desalting of plasmid DNA by centrifugation
| Anwendung | Konzentrierung und Entsalzung von Plasmid-Eluaten |
|---|---|
| Automatische Nutzung | Nein |
| CE-zertifiziert | Nein, nur für wissenschaftlichen Gebrauch |
| Elutionsvolumen | ≥ 100 µL |
| Format | Trichtersäule |
| Gefahrstoffe | Nein |
| Haltbarkeit (ab Produktion) | 27 Monat(e) |
| Handhabung | Zentrifugation |
| Lagertemperatur | 15–25 °C |
| Präparationszeit | 15 min/6 Präps |
| Probenmaterial | Bakterien, E. coli |
| Probenmenge | DNA-Eluat |
| Technologie | Fällung und Filtration |
| Theoretische Bindungskapazität | 1.5 mg |
| Typische Downstream-Anwendung | Transfektion von empfindlichen Zellen und Zellinjektionen |
| Typische Rückgewinnung | 90−100 % |
| Vektorgröße | < 25 kbp |
| Verkaufseinheit | 10 Präp(s), 50 Präp(s) |
| Warenzeichen | NucleoSpin |
| Ziel | Plasmid DNA |
Plasmid DNA
Desalting and Concentraction
Following the elution of pure plasmid DNA from the NucleoBond columns, MN provides a range of options for desalting and concentrating the plasmid. In addition to the standard isopropanol precipitation followed by centrifugation we offer the NucleoBond Finalizers for manual desalting with a syringe as well as centrifuge-compatible NucleoSpin Finisher and vacuum compatible NucleoSnap Finisher for a fast, simple, and easy prep.
Isopropanol precipitation
(traditional method, duration 1 h)
NucleoBond Finalizer and Finalizer Large (ideal for small number of samples, duration 5 min/sample)
NucleoBond Finalizer Finalizer Large
NucleoSpin Finisher (for parallel processing of samples, with appropriate centrifuge, duration < 10 min/12 samples)
NucleoSpin Finisher
NucleoSnap Finisher Midi and Maxi (for parallel processing of samples, vacuum manifold, duration < 10 min/12 samples)
NucleoSnap Finisher Midi NucleoSnap Finisher Maxi
NucleoMag Desalting Beads (can be automated on the Andrew+ in combination with NucleoBond Xtra Midi kits)
NucleoMag Desalting Beads
NucleoBond Finalizer
The NucleoBond Finalizers are designed to concentrate and desalinate plasmid DNA eluates collected using NucleoBond columns after anion-exchange chromatographic DNA purification. The sample is syringe-loaded onto a NucleoBond Finalizer membrane to avoid tedious centrifugation steps.
NucleoSpin and NucleoSnap Finisher
NucleoSpin Finisher are designed for quick concentration and desalting of plasmid eluates obtained by anion-exchange chromatography. Finisher replace tedious and time-consuming isopropanol precipitation. Furthermore, problems like lost DNA pellets or incomplete solubilization of hardly visible precipitates can easily be circumvented.
NucleoMag Desalting Beads
NucleoMag Desalting beads are designed for quick concentration and desalination of plasmid DNA eluates that are obtained be anion exchange chromatographic plasmid purification kits. Plasmid DNA is bound reversibly to NucleoMag Desalting beads followed by ethanolic wash steps. Finally pure plasmid DNA is eluted using a low salt buffer. NucleoMag Desalting beads can be automated on e.g the Andrew+ platform.
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