NucleoSpin 96 Blood Core Kit, 96-well Kit für DNA aus Blut,
Preis auf Anfrage
*ohne Steuern und Versandkosten
Versatile purification of high quality DNA from whole blood in proven 96‑well plate format
| Anwendung | Isolierung von DNA |
|---|---|
| Automatische Nutzung | Ja |
| CE-zertifiziert | Nein, nur für wissenschaftlichen Gebrauch |
| Elutionsvolumen | 100 µL |
| Format | 96-Well-Platte |
| Fragmentgröße | 300 bp–ca. 50 kbp |
| Gefahrstoffe | Ja |
| Gewicht Brutto (ink. Verpackung) | 1874.8 g |
| Haltbarkeit (ab Produktion) | 19 Monat(e) |
| Handhabung | Zentrifugation, Vakuum |
| Kompatibilität der Automatisierung | Kompatibel mit den meisten offenen Roboterplattformen. Bitte besuchen Sie www.mn-net.com/automation oder wenden Sie sich an unseren technischen Support für weitere Informationen. |
| Lagertemperatur | 15–25 °C |
| Lieferumfang | NucleoSpin Blood Binding Plates (4 x 96), buffers, Proteinase K |
| Präparationszeit | 70 min/Platte |
| Probenmaterial | Blut, Zellen |
| Probenmenge | 200 µL Vollblut (menschlich oder tierisch, frisch oder gefroren, behandelt mit Citrat, EDTA, Heparin oder CPDA), 2x10⁶ kultivierte Zellen |
| Technologie | Silica-Membrantechnologie |
| Theoretische Bindungskapazität | 20 µg |
| Typische Ausbeute | 4–6 µg |
| Typische Downstream-Anwendung | Next Generation Sequenzierung, PCR, Southern Blotting, enzymatische Reaktionen |
| Typische Reinheit A260/A280 | 1,8–1,9 |
| Verpackungsabmessung | 220 x 220 x 230 mm |
| Warenzeichen | NucleoSpin |
| Ziel | DNA |
NucleoSpin96 Blood
- Time-saving parallel isolation of genomic DNA from whole blood
- Optimized for manual or automated use under vacuum
- Improved flow-rate reduces risk of clogging – no cross-contamination, reliable results
- Innovative MN Wash Plate minimizes risk of cross-contamination
- Complete processing possible at room temperature
- Consistently high yields
NucleoSpin96 Blood automated use with the epMotion 5075
NucleoSpin96 Blood is designed for the parallel isolation of genomic DNA from whole blood. After lysis of blood cells genomic DNA is selectively bound to a silica membrane while contaminants are washed away effectively. Elution is possible even under vacuum without cross-contamination. Due to its plate design and the possibility to perform the complete procedure under vacuum NucleoSpin96 Blood can be easily adapted to common liquid handling instruments for fully automated processing without manual interactions.
Yield and purity of DNA - Reproducibility of DNA purification
gDNA was isolated from a pool of human blood samples stored at 4 °C. 200 µL of human blood were used for DNA purification. DNA yield and purity were determined by UV measurement.
Applications Notes available
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