NucleoMag Dx Pathogen, CE-IVD-zertifizierte Isolierung viraler RNA/DNA
Preis auf Anfrage
*ohne Steuern und Versandkosten
Auf magnetischen Beads basiertes Kit für die Isolierung viraler RNA aus humanem Speichel und Nasen-Rachen-Abstrichen (validiert für SARS-CoV-2), sowie für die Isolierung viraler RNA und viraler DNA aus humanen Stuhlproben (validiert für Norovirus). Konform mit IVDR 2017/746.
| Anwendung | Isolierung von viralen Nukleinsäuren |
|---|---|
| Automatische Nutzung | Ja |
| CE-zertifiziert | Ja, CE-IVD-gekennzeichnet für die diagnostische Verwendung in der EU und anderen Ländern |
| Elutionsvolumen | 50–100 µL |
| Format | Magnetic beads |
| Fragmentgröße | 300 bp–ca. 50 kbp |
| Gefahrstoffe | Ja |
| Gewicht Brutto (ink. Verpackung) | 1897 g |
| Haltbarkeit (ab Produktion) | 19 Monat(e) |
| Handhabung | Magnetische Trennung |
| Kompatibilität der Automatisierung | Kompatibel mit den meisten offenen Roboterplattformen. Bitte besuchen Sie www.mn-net.com/automation oder wenden Sie sich an unseren technischen Support für weitere Informationen. |
| Lagertemperatur | 18–25 °C |
| Präparationszeit | 40-120 min/96 Präps, je nach verwendeten Geräten/Automaten |
| Probenmaterial | Human Nasen-Rachen-Abstrichen, Humane Stuhlproben, Humaner Speichel |
| Probenmenge | ≤ 200 µL Tupferwaschlösung/Speichel/Stuhlsuspension |
| Technologie | Magnetische Bead-Technologie |
| Theoretische Bindungskapazität | 0.4 µg/µL beads |
| Typische Downstream-Anwendung | NGS, RT−PCR |
| Typische Rückgewinnung | Abhängig von Probenmenge und Qualität |
| Verfügbarkeit | Für US-Kunden bestellen Sie bitte das äquivalente NucleoMag Pathogen, REF 744210.4 (nur für Forschungszwecke) |
| Verpackungsabmessung | 220 x 220 x 234 mm |
| Warenzeichen | NucleoMag |
| Ziel | Viral RNA und DNA |
NucleoMag Dx Pathogen*
- CE-IVD-certified – compliant with European IVD Regulation 2017/746
- Suitable for viral RNA isolation from human respiratory swabs and saliva
- Suitable for viral RNA and viral DNA from human stool samples
- Magnetic bead based purification - manual or automated (open access platforms)
- Excellent diagnostic sensitivity
The NucleoMag Dx Pathogen kit is intended for the isolation of viral RNA from human respiratory samples, including nasal and oral swabs as well as saliva. Furthermore, the kit is intended for viral RNA and viral DNA from human raw stool samples. The purification of viral nucleic acid is based on the reversible binding of nucleic acids to the NucleoMag magnetic beads. The NucleoMag Dx Pathogen can be automated on common open access automation platforms.
The kit is available as IVD product compliant with European IVD Regulation, as research-use only product (REF 744210.1/.4/.100), and as prefilled plates for convenient automated use (REF 744211). These kits include ready to use buffers, Proteinase K and Carrier RNA.
The NucleoMag Dx Pathogen kit shows market-leading performance enabling reliable detection of RNA and DNA viruses from clinical samples.
Application data
Excellent diagnostic sensitivity – competitor comparison
Consistent performance with different sample types
Efficient isolation of viral nucleic acids
Sensitivity screening for pathogen detection from human stool samples
References
Publication
Sample type – Nucleic acid
Tran, X.D., Hoang, V.T., Dao, T.L. et al.
High Prevalence of Non-typeable Haemophilus influenzae and Haemophilus haemolyticus Among Vaccinated Children with Community-Acquired Pneumonia in Vietnam.
J Epidemiol Glob Health 14, 498–501 (2024).
- Nasopharyngeal samples
- DNA and RNA extraction
- Automated on Thermo Scientific KingFisherTM* Flex
Beaufils, Q., Bénéjat, L., Ducournau, A. et al.
Performance evaluation of the Biosynex AMPLIQUICK Fecal Bacteriology PCR kit.
BMC Microbiol 25, 400 (2025).
- Stool samples – 200µL Fecal SwabTM* liquid
- Bacterial DNA extraction
- Automated on MagnetaPure
Charfi, R., Guyonnet, C., Untrau, M. et al.
Performances of two rapid LAMP-based techniques for the intrapartum detection of Group B Streptococcus vaginal colonization.
Ann Clin Microbiol Antimicrob 23, 37 (2024).
- Cell culture from vaginal swab sample
- Nucleic acid extraction
Tran, X.D., Hoang, VT., Goumballa, N. et al.
Viral and bacterial microorganisms in Vietnamese children with severe and non-severe pneumonia.
Sci Rep 14, 120 (2024).
- Nasopharyngeal samples + Sigma-Transwab®* medium
- Viral DNA & viral RNA extraction
- Automated on Thermo Scientific KingFisherTM* Flex
Tran, X.D., Hoang, V.T., Dang, T.T.D. et al.
Aetiology of Acute Undifferentiated Fever Among Children Under the Age of Five in Vietnam: A Prospective Study.
J Epidemiol Glob Health 13, 163–172 (2023).
- Blood samples
- Viral DNA & viral RNA extraction
- Automated on Thermo Scientific KingFisherTM* Flex
Script support for automation
Basic scripts or protocols for the implementation of the NucleoMag Dx Pathogen kit on different open access automation platforms can be provided by MACHEREY-NAGEL.
If the NucleoMag Dx Pathogen kit is used in in-vitro diagnostic workflows, the use of the kit must be validated by the user in conjunction with the automation platform and consumables used and the subsequent in-vitro diagnostic assay (e.g., qRT-PCR). It is recommended to use appropriate controls (e.g. internal controls, extraction controls, positive / negative controls).
Individual protocol steps may vary depending on available consumables, hardware, platform and instrument setup.
Click here to read about the MACHEREY-NAGEL script support for NucleoMag Dx Pathogen.
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