MN Bead Tube Type D
Homogenization of difficult to lyse tissues or even insects or microbes within tissues is a challenging lyis approach when purifying nucleic acids. The MN Bead Tubes Type D are 3 mm steel beads that are able to overcome these challenges.
Insects, crustaceans, lipid-rich tissues
Type D (3 mm steel) 3 mm steel beads
Compatible with the following kits
Scalable options
Solutions for small sample amounts
- 2 mL screw cap tubes
- Prefilled with 3 mm steel beads
- compatible with common bead beating devices
- click here for more product information
Bulk solutions for different sample amounts
- bulk beads
- DNA and nuclease free
- 3 mm steel beads
- compatible with common bead beating devices
- click here for more information
HTP solution
- Pack of prefilled tube strips (8x12)
- Prefilled with 3 mm steel beads
- compatible with common plate disruption device
- click here for more product information
Sample processing recommendations
In order to obtain optimal yields of DNA from sample material, a complete disruption of the sample material is necessary. Sample disruption efficiency depends on the parameters such as type of bead tube or bead plate, time and frequency and also the discrution device. Please find below our suggested recommendations for the MN Bead Tubes Type D in combination with different nucleic acid purifiaction kits.
MN Bead Tube Holder in combination with a Vortex-Genie 2
Sample disruption using the MN Bead Tube Holder is a very straight forward and easy method to homogenize your sample. The MN Bead Tube Holder is available for 2 mL and 5 mL bead tubes.
| Sample type | Processing time | Buffer and volume | Recommended kit |
| Brain tissue (fresh or frozen) | Approx. 20 min full speed, RT | according to the standard protocol | NucleoSpin DNA Lipid Tissue |
| Adipose tissue (fresh or frozen) | Approx. 20 min full speed, RT | according to the standard protocol | NucleoSpin DNA Lipid Tissue |
| Fatty fish tissue (fresh or frozen) | Approx. 20 min full speed, RT | according to the standard protocol | NucleoSpin DNA Lipid Tissue |
| Insect material (Drosophila melanogaster) | Approx. 0.5-15 min, full speed, RT | according to the standard protocol | NucleoSpin DNA Insect |
Sample processing recommendation using a RETSCH Mixer Mill
An alternative in sample disruption of biological materials are bead beating mills. MN Bead Tubes (with the exception of MN Bead Tubes Type F) can be combined with most disruption devices. Please find below the recommendation for MN Bead Tubes D incombination with different purification kits and the RETSCH Mixer Mill. Click here to find our latest Application Note for MN Bead Tubes and the RETSCH Mixer Mill.
MN Bead Tubes Type D
| Sample type | Processing time | Buffer and volume (supplementary) | Bead Tube | Recommended kit |
| Soft tissue (brain, liver, adipose or fatty fish tissue) | 1 min at 10 Hz followed by 10 s at 20 Hz | <40 mg tissue + 40 µL LBP + 100 µL IME + 10 µL ProK + 2.5 µL RNase A | MN Bead Tubes Type D | NucleoMag DNA Bacteria |
| Insects (fresh, frozen, ethanol preserved) | 0.5-10 min at 30 Hz | Insect + 100 µL BE + 40 µL MG, 10 µL Proteinase K | MN Bead Tubes Type D | NucleoSpin DNA Insect |
| Lipid rich tissue | 0.5-10 min at 30 Hz |
according to the standard protocol
| MN Bead Tubes Type D | NucleoSpin DNA Lipid Tissue |
| Filamentous fungi (Aspergillus nidulans, melon mold, citrus mold, Potato mold) | 1-2 min at 25 Hz | <40 mg cell pellet+ 100 µL BE+ 40 µL MG + 10 µL Proteinase | MN Bead Tubes Type D | NucleoMag DNA Bacteria |
MN 96 Bead Plate D
| Sample type | Processing time | Buffer and volume (supplementary) | Bead Tube | Recommended kit |
| Soft tissue (brain, liver, adipose or fatty fish tissue) | 1 min at 10 Hz followed by 10 s at 20 Hz | <40 mg tissue + 40 µL LBP+ 100 µL IME + 10 µL Proteinase K + 2.5 µL RNase A | MN 96 Bead Plate Type D | NucleoMag DNA Bacteria |
| Filamentous fungi (Aspergillus nidulans, Meldon mold, citrus mold, potato mold) | 2 x 1 min at 20 Hz | <40 mg microbial pellet + 100 µL BE + 40 µL MG + 10 µL Proteinase K | MN 96 Bead Plate Type D | NucleoMag DNA Bacteria |