Analysis of milk

Nucleic acid extraction from milk and dairy products

Tracking genetically modified organisms (GMOs) strengthens consumer confidence in milk and dairy products and ensures that they comply with national and international regulations. Molecular biological methods such as qPCR, PCR or sequencing can detect even small amounts of GMOs. Molecular biological detection methods also play a decisive role in the detection of pathogens or germs (Salmonella spp., Campylobacter spp., Listeria, molds, etc.), in identification and species detection or in origin analysis. MACHEREY-NAGEL's NucleoSpin VET and NucleoMag VET extraction kits are the ideal solutions for your molecular biological milk analysis. These kits offer a solution for the manual or automated high-throughput extraction of highly pure nucleic acids from milk samples.

DNA extraction from cartilage and bulk milk samples

Milk somatic cells are a mixture of milk-producing cells and immune cells and are secreted in milk during the normal milking process. The somatic cell count (SCC) can be used to detect infections in the animal herd at an early stage, thus providing a simple tool for herd management.

Learn more about how MACHEREY-NAGEL's NucleoSpin 96 Tissue kit can be used to monitor bulk tank milk samples in our application note on this innovative technique.The technique uses the NucleoSpin 96 Tissue kit for automated DNA extraction on a TECAN Fluent Automation Workstation. The purified DNA can be used for genotyping in order to match the genotype of the herd animals with the presence of their DNA in the bulk milk sample (somatic cell count). SCCs are widely used as a marker to monitor the prevalence of mastitis in dairy herds, as an indicator of raw milk quality to processors, and also as a more general indicator of the hygienic conditions of milk production on farms.

Automated Nucleic Acid Extraction / HTP

Automation is an important building block to standardize your molecular biology workflows in the laboratory and to obtain better consistency and reliability of your results even when working with heterogeneous, complex food matrices. MACHEREY-NAGEL's NucleoMag VET extraction kit has been developed with hands-off in mind and is an optimized solution for automated nucleic acid extraction. The extracted nucleic acids are free of inhibitors, have optimal quality and are therefore ideally suited for all common assays such as quantitative real-time PCR (qRT-PCR) or sequencing. Benefit from our automation expertise and get a reproducible, simple and effective method to cope with the increasing number of samples in milk diagnostics and hygiene monitoring.