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NucleoSpin Plasmid (NoLid) Columns for plasmid DNA purification

Rapid Mini preparation of plasmid DNA with columns without lid

Application High and low copy plasmid DNA purification
Automated use No
Brand NucleoSpin
CE certified No, research use only
Elution volume 50 µL
Endotoxin level > 50 EU/µg DNA
Format Mini prep
Handling Centrifugation, vacuum
Hazardous material No
Lysate clarification Centrifugation
Preparation time 20 min/6 preps
Sample amount 1−5 mL (high copy), 6−10 mL (low copy)
Sample material Bacteria, E. coli
Selling unit 50 Piece(s), 250 Piece(s)
Shelf life (from production) 27 Month(s)
Storage temperature 15–25 °C / 59–77 °F
Target Plasmid DNA
Technology Silica membrane technology
Theoretical binding capacity 60 µg
Typical downstream application Transfection of sensitive cells and cell injections
Typical purity A260/A280 1.8−1.85
Typical yield 25−45 µg
Vector size < 25 kbp

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Accessories

Resuspension Buffer A1
Resuspension Buffer A1

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REF 740911.1
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Wash Buffer AW
Wash Buffer AW

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REF 740916.1
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Neutralization Buffer A3
Neutralization Buffer A3

Content 1000 mL

REF 740913.1
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Buffer A4 Concentrate
Buffer A4 Concentrate

Content 200 mL

REF 740914.1
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Lysis Buffer A2 (without LyseControl)
Lysis Buffer A2 (without LyseControl)

Content 1000 mL

REF 740912.1
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NucleoSpin Plasmid Buffer Set
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Content 1 Set(s)

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