NucleoSpin Plasmid EasyPure, Mini kit for easy plasmid DNA purification
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NucleoSpin Plasmid EasyPure is a spin column kit based for manual use. It has been developed for the rapid isolation of plasmid DNA from bacteria such as Escherichia coli. The innovative buffer design enables a one-step washing procedure, allowing isolation in under 10 minutes. Plasmid DNA is eluted in a slight alkaline buffer (5 mM Tris/HCl, pH 8.5), thus being ready-to-use for subsequent applications.
| Application | High copy plasmid DNA purification |
|---|---|
| Automated use | No |
| Brand | NucleoSpin |
| CE certified | No, research use only |
| Elution volume | 50 µL |
| Endotoxin level | > 50 EU/µg DNA |
| Format | Mini prep |
| Handling | Centrifugation, vacuum |
| Hazardous material | Yes |
| Lysate clarification | Centrifugation |
| Preparation time | 14 min/6 preps |
| Sample amount | 1−5 mL |
| Sample material | Bacteria, E. coli |
| Selling unit | 10 Prep(s), 50 Prep(s), 250 Prep(s) |
| Shelf life (from production) | 27 Month(s) |
| Storage temperature | 15–25 °C / 59–77 °F |
| Target | Plasmid DNA |
| Technology | Silica membrane technology |
| Theoretical binding capacity | 35 µg |
| Typical downstream application | Cloning, PCR, Restriction analysis, Sequencing, Transformation |
| Typical purity A260/A280 | 1.8−1.85 |
| Typical yield | 15−30 µg |
| Vector size | < 25 kbp |
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