NucleoSpin Gel and PCR Clean-up, Mini kit for gel extraction or PCR clean up
Price on request
*taxes and shipping not included
NucleoSpin Gel and PCR Clean-up is a spin column based kit providing a 2-in-1 solution for the purification of nucleic acids from both PCR mixtures and gels. The kit has been designed with various contaminants and variable sample volumes in mind, ensuring optimal recovery and purity of each individual sample.
| Application | PCR clean up and gel extraction |
|---|---|
| Automated use | No |
| Brand | NucleoSpin |
| CE certified | No, research use only |
| Elution volume | 15−30 µL |
| Format | Mini prep |
| Fragment size | < 25 kbp |
| Handling | Centrifugation, vacuum |
| Hazardous material | Yes |
| Preparation time | 10 min/6 preps |
| Sample amount | < 200 µL PCR reaction mixture, < 200 mg agarose gel |
| Sample material | Agarose gel, PCR reaction mixture |
| Selling unit | 10 Prep(s), 50 Prep(s), 250 Prep(s) |
| Shelf life (from production) | 27 Month(s) |
| Storage temperature | 15–25 °C / 59–77 °F |
| Target | Clean up |
| Technology | Silica membrane technology |
| Theoretical binding capacity | 25 µg |
| Typical downstream application | Cloning, PCR, Restriction analysis, Sequencing |
| Typical purity A260/A280 | 1.8−1.9 |
| Typical recovery | 70−95 % |
In photometry, it is important to detect interferences so that measurement results can be evaluated correctly. The composition of the tested samples depends strongly on the upstream processes. When using our test kits, we recommend checking for possible sources of interference in the sample. In this seminar, we will show you which interferences can occur, how to recognize them and how these interferences can be eliminated or avoided. Among other things, we will cover the important industrial parameters COD, Phosphate, Nitrate and Nitrit in theory and practice. The presentation language will be german. Content: General information on IQC Pipette control and proper pipetting Carrying out various practical exercises Inspection equipment monitoring of the photometer Date/Location: April 10, 2025, 9:00 a.m. - 3:30 p.m. CET Wasserburg am Inn (DE) Registration: Please register here for this seminar. The seminar is free of charge for all participants. Deadline for registration is one week before seminar date. General information: The seminar is free of charge and independent of which measuring system you use Drinks, coffee breaks and lunch will be provided Participants will receive detailed seminar documentation and a certificate of attendance Registrations will be considered at the latest one week before the seminar date The number of participants is limited. Registrations will be considered according to the date of receipt.
The NANOCOLOR Advance facilitates the evaluation of all photometrically assessable MACHEREY-NAGEL test kits, including:
- VISOCOLOR ECO
- VISOCOLOR Powder Pillows
- NANOCOLOR ECO
- NANOCOLOR tube tests
- NANOCOLOR standard tests
With its diverse range of wavelengths, the NANOCOLOR Advance not only supports rapid tests from the MACHEREY-NAGEL portfolio but also enables various special applications with COD. In addition to numerous preprogrammed methods, users have access to a menu for programming customized applications.
Preprogrammed methods include:
- Nephelometric turbidity measurement; 860 nm; 1 – 1000 NTU
- Formazin Attenuation Units; 550 nm; 1 – 100 FAU
- Formazin Attenuation Units; 860 nm; 2 – 400 FAU
- Spectral absorption coefficient 436 nm; 0.1 – 150 1/m
- Total Suspended Solids (TSS); 800 nm; 5 – 750 mg/L
- Color APHA 2120 C/Hazen; 455 nm; 5 – 500 mg/L Pt
- Color ISO 7887B; 436, 520, 620 nm; 0.2 – 20 1/m
- Color ISO 7887C; 410 nm; 5 – 500 mg/L Pt
User-specific programming options are:
- Single wavelength measurement with factor input
- Multi-wavelength measurement, including calculation and factor input
- Method calibration through absorbance-concentration value pairs
- Calibration method by measuring concentration series
Our NANOCOLOR round cuvette tests for the determination of CB, Ammonium, Nitrate and Total Nitrogen comply with ISO standards. These standards were specifically developed for the determination of water quality using small, sealed vessels.
In particular, these are:
1) ISO 15705:2002: Water quality — Determination of chemical oxygen demand (ST-COD) — Small-scale technique in sealed vessels. This standard specifies the determination of CB in any aqueous sample, including all wastewater and pollutant water.
2) ISO 23695:2023-02: Water quality — Determination of ammonium nitrogen in water — Small-scale technique in sealed vessels. This standard specifies the determination of Ammonium in drinking water, groundwater, surface water, wastewater, bathing water and mineral water with measurement ranges from 0.01–1800 mg/L NH₄-N.
3) ISO 23696-1:2023-02: Water quality — Determination of nitrates in water with small-scale sealed vessels — Part 1: Dimethylphenol colorimetric method. This standard specifies the determination of Nitrates in natural waters, drinking water and wastewater with measurement ranges from 0.10–225 mg/L NO₃-N.
4) ISO 23697-1:2023-02: Water quality — Determination of total bound nitrogen (ST-TNb) in water with small-scale sealed vessels — Part 1: Dimethylphenol colorimetric method. This standard specifies the determination of Total Nitrogen in groundwater, surface water and wastewater with measurement ranges from 0.5–220 mg/L N.
These standards provide clear guidelines and procedures for the precise measurement of chemical oxygen demand and various nitrogen parameters in water samples.
They are of great importance for environmental experts, laboratories and companies dealing with water treatment. By complying with these standards, our customers can ensure that our NANOCOLOR test kits are precise, reliable and comparable.
Determination of the chemical oxygen demand index (ST-COD)
This standard method specifies the determination of COD in any aqueous samples
Our NANOCOLOR round cuvette tests for the determination of ST-COD, Ammonium, Nitrat, and total bound nitrogen comply with ISO standards. These standards were specifically developed for the determination of water quality with small, closed vessels.
Specifically, these are:
1) ISO 15705:2002: Water quality — Determination of chemical oxygen demand (ST-COD) — Small-scale technique with closed vessels
This standard specifies the determination of ST-COD in any aqueous sample, including all wastewater and pollutant waters.
2) ISO 23695:2023-02: Water quality — Determination of ammonium in water — Small-scale technique with closed vessels
This standard specifies the determination of Ammonium in drinking water, groundwater, surface water, wastewater, bathing water, and mineral water with measurement ranges of 0.01–1800 mg/L NH₄-N.
3) ISO 23696-1:2023-02: Water quality — Determination of nitrates in water with small-scale closed vessels — Part 1: Dimethylphenol colorimetric test
This standard specifies the determination of Nitrat in natural waters, drinking water, and wastewater with measurement ranges of 0.10–225 mg/L NO₃-N.
4) ISO 23697-1:2023-02: Water quality — Determination of total bound nitrogen (ST-TNb) in water with small-scale closed vessels — Part 1: Dimethylphenol colorimetric test
This standard specifies the determination of ST-TNb in groundwater, surface water, and wastewater with measurement ranges of 0.5–220 mg/L N.
These standards provide clear guidelines and procedures for the precise measurement of ST-COD and various nitrogen parameters in water samples. They are of great importance for environmental experts, laboratories, and companies involved in water treatment. By adhering to these standards, our customers can ensure that our NANOCOLOR test kits are precise, reliable, and comparable.
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