NucleoSpin Blood L, Midi kit for DNA from blood using vacuum processing
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NucleoSpin Blood L Vacuum is a column based kit for the manual and automated isolation of genomic DNA from up to 2 mL blood samples using vacuum processing . It is intended for the isolation of DNA from blood samples stabilized with common anticoagulants. Besides medium sized columns it contains buffers and proteinase K.
| Application | Isolation of DNA |
|---|---|
| Automated use | No |
| Brand | NucleoSpin |
| CE certified | No, research use only |
| Elution volume | 2 x 300 µL |
| Format | Midi prep |
| Fragment size | 200 bp–approx. 50 kbp |
| Gross weight (incl. packaging) | 892.4 g / 1.97 lbs |
| Handling | Vacuum |
| Hazardous material | Yes |
| Packaging dimensions | 233 x 161 x 136 mm / 9.17 x 6.34 x 5.35 Inch |
| Preparation time | 75 min/24 preps |
| Sample amount | 1–2 mL |
| Sample material | Blood |
| Scope of delivery | NucleoSpin Blood L Columns, buffers, Proteinase K |
| Shelf life (from production) | 24 Month(s) |
| Storage temperature | 15–25 °C / 59–77 °F |
| Target | DNA |
| Technology | Silica membrane technology |
| Theoretical binding capacity | 250 µg |
| Typical concentration | 100–130 ng/µL |
| Typical downstream application | Next Generation Sequencing, PCR, Southern blotting, enzymatic reactions |
| Typical purity A260/A280 | 1.6–1.9 |
| Typical yield | 50–80 µg (2 mL blood) |
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