NucleoMag RNA Blood for RNA Isolation from blood
Price on request
*taxes and shipping not included
The NucleoMag RNA Blood Kit is intended for the purification of RNA from whole blood samples collected in EDTA / Citrate blood collection tubes or in SARSTEDT® S-Monovette RNA Exact, Zymo DNA/RNA Shield™ Blood Tubes, or Tempus™ RNA Blood Tubes. With a focus on simplicity and efficiency, this kit eliminates the need for reagent removal and pelleting*, ensuring smooth processing. The purified RNA obtained from this kit is suitable for a range of downstream applications including RT-PCR and sequencing. The kit can be used either manually or automated on standard liquid handling instruments or automated magnetic separators. Included in the kit are essential components such as NucleoMag B-Beads, Buffers, Liquid Proteinase K, and RNase-free rDNase, ensuring reliable and high-quality RNA extraction. Note: Blood collection and stabilization tubes are not provided and must be purchased separately. *Processing of EDTA / Citrate or Tempus RNA Blood Tubes necessitates additional equipment and buffers as detailed in the user manual for comprehensive guidance.
| Application | Isolation of RNA from whole blood |
|---|---|
| Automated use | Yes |
| Automation support | Compatible with most open robotic platforms. Please visit www.mn-net.com/automation or consult our technical support for further information. |
| Brand | NucleoMag |
| CE certified | No, research use only |
| Elution volume | 50–100 µL |
| Format | Magnetic beads |
| Fragment size | > 200 nt |
| Handling | Magnetic separation |
| Hazardous material | Yes |
| Preparation time | Approx. 90 min for automated protocol, Approx. 120 min for manual protocol |
| Sample amount | 9 mL of stabilized blood from Tempus™ Blood RNA Tube (corresponds to 3 mL whole blood), 260 µL EDTA / Citrate blood, 820 µL of stabilized blood from SARSTEDT S-Monovette® RNA Exact (corresponds to approx. 200 µL whole blood), 820 µL stabilized blood from Zymo Research DNA/RNA Shield™ Blood Collection Tube (corresponds to approx. 270 µL whole blood) |
| Sample material | Whole blood collected in SARSTEDT S-Monovette® RNA Exact, Zymo DNA/RNA Shield Blood Tubes, Tempus RNA Blood Tubes, or EDTA / Citrate blood collection tubes |
| Selling unit | 96 Prep(s), 384 Prep(s) |
| Shelf life (from production) | 24 Month(s) |
| Storage temperature | 15–25 °C / 59–77 °F |
| Target | RNA |
| Theoretical binding capacity | 0.4 µg/µL beads |
| Typical downstream application | Microarray spotting, NGS, Next Generation Sequencing, RNA-Seq, RT−PCR, cDNA library construction, other enzymatic reactions, qPCR |
| Typical RIN | ≥ 6-10 (strongly depends on sample storage and quality) |
| Typical yield | 3-4 µg (strongly depends on sample quality and health status of donor) |
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