NucleoMag Plasmid, kit for plasmid DNA isolation with magnetic beads
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The NucleoMag Plasmid Kit is ideal for automated isolation of plasmid DNA on magnetic rod systems such as MagnetaPure 32 Plus or IsoPure Mini. Typical downstream applications with the isolated plasmid DNA are sequencing, cloning, PCR, transformation, transfection, in vitro transcription, ligation.
| Application | High copy plasmid DNA purification |
|---|---|
| Automated use | Yes |
| Brand | NucleoMag |
| CE certified | No, research use only |
| Elution volume | 50–200 µL |
| Endotoxin level | ≤ 50 EU/µg DNA - Lysate clearance via magnetic beads, ≤ 50 EU/µg DNA - Lysate clearance via centrifugation (3 wash steps), ≤ 10 EU/µg DNA - Lysate clearance via centrifugation (4 wash steps) |
| Format | Magnetic beads |
| Fragment size | < 25 kbp |
| Handling | Automated |
| Hazardous material | Yes |
| Lysate clarification | Centrifugation, Magnetic Beads |
| Sample amount | ≤ 5 mL E.coli culture |
| Sample material | Bacteria, E. coli |
| Selling unit | 96 Preps, 384 Preps |
| Shelf life (from production) | 15 Month(s) |
| Storage temperature | 15–25 °C / 59–77 °F |
| Target | Plasmid DNA |
| Technology | Magnetic bead technology |
| Typical downstream application | Cloning, In-vitro transcription, PCR, Restriction analysis, Sequencing, Transfection of cells, Transformation |
| Typical purity A260/A230 | ≥ 2.0 |
| Typical purity A260/A280 | ≥ 1.8 |
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