NucleoBond AX 100, Midi columns for transfection-grade plasmid DNA
Price on request
Package unit
100 Piece(s)
*taxes and shipping not included
Item number:
740521.100
Package unit
100 Piece(s)
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1st generation anion exchanger for purification of plasmid DNA
| Application | High and low copy plasmid DNA purification |
|---|---|
| Automated use | No |
| Brand | NucleoBond |
| CE certified | No, research use only |
| Endotoxin level | 1−10 EU/µg DNA |
| Format | Midi prep |
| Handling | Gravity flow |
| Hazardous material | No |
| Isopropanol precipitation | Centrifugation, NucleoBond Finalizer, NucleoSnap Finisher Midi, NucleoSpin Finisher Midi |
| Lysate clarification | Folded filter |
| Preparation time | 65 min/4−6 preps |
| Sample amount | 5−30 mL (high copy), 10−100 mL (low copy) |
| Sample material | Bacteria, E. coli |
| Selling unit | 20 Piece(s), 100 Piece(s) |
| Shelf life (from production) | 72 Month(s) |
| Storage temperature | 15–25 °C / 59–77 °F |
| Target | Plasmid DNA |
| Technology | Anion exchange chromatography |
| Theoretical binding capacity | 100 µg |
| Typical downstream application | Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells |
| Typical purity A260/A280 | 1.8−1.95 |
| Typical yield | 20−100 µg |
| Vector size | < 300 kbp |
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