MN Bead Tube Type B
Cultured microorganisms such as bacteria are complex samples that often require mechanical homogenization to ensure a complete lysis. Our MN Bead Tubes Type B include glass beads in a mixture of sizes (40 - 400 um) and are an efficient tool for effective homogenization of cultured bacterial samples.
Bacteria
Type B (glass) 40 - 400 µm glass beads
Compatible with the following kits
Scalable options
Solutions for small sample amounts
- 2 ml screw cap tubes
- Prefilled with 40-400 µm glass beads
- compatible with common bead beating devices
- click here for more information
Bulk solutions for different sample amounts
- bulk beads
- DNA and nuclease free
- 40-70 µm glass beads
- recommended to be mixed in a ratio of 1:5 with B2 bulk glass beads
- compatible with common bead beating devices
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- bulk beads
- DNA and nuclease free
- 30 - 400 µm glass beads
- recommended to be mixed in a ratio of 5:1 with B1 bulk glass beads
- compatible with common bead beating devices
- click here for more information
HTP solution
- Pack of prefilled tube strips (8x12)
- Prefilled with 40-400 µm glass beads
- compatible with common plate disruption device
- click here for more information
Sample processing recommendations
In order to obtain optimal DNA yields from sample materials, a complete disruption of the sample is necessary. Sample disruption efficiency depends on several parameters such as the type of bead tube or plate, time and frequency of disruption, and also the disruption device.
Please see below for our suggested recommendations for implementing the MN Bead Tubes Type B with various nucleic acid purification kits:
MN Bead Tube Holder in combination with a Vortex-Genie 2
Sample disruption using the MN Bead Tube Holder is a very straight forward and easy method to homogenize your sample. The MN Bead Tube Holder is available for 2 ml and 5 ml bead tubes.
| Sample type | Processing time | Buffer and volume | Recommended kit |
| Microbial samples | Approx. 20 min full speed, RT | according to the standard protocol | NucleoSpin Microbial DNA |
Sample processing recommendation using a RETSCH Mixer Mill
MN Bead Tubes (with the exception of MN Bead Tubes Type F) can be combined with most disruption devices. Please find below the recommendation for MN Bead Tubes Type B in combination with different purification kits and the RETSCH Mixer Mill. Click here to find our latest Application Note for MN Bead Tubes and the RETSCH Mixer Mill.
MN Bead Tubes Type B
| Sample type | Processing time | Buffer and volume (supplementary) | Bead Tube | Recommended kit |
| Gram negative bacteria (Escherichia Coli, Vibro fischeri | 4-6 min, 30 Hz | <40 mg microbial pellet + 140 µl BE, 10 µl Proteinase K, 2.5 µl RNaseA | MN Bead Tubes Type B | NucleoSpin Microbial DNA |
| Gram positive bacteria (Bacillus subtilis, Corynebacterium glutamicum) | 12 min, 30 Hz | <40mg microbial pellet + 140 µl BE, 10 µl Proteinase K, 2.5 µl RNaseA | MN Bead Tubes Type B | NucleoSpin Microbial DNA |
MN 96 Bead Plate Type B
| Sample type | Processing time | Buffer and volume (supplementary) | Bead Tube | Recommended kit |
| Gram negative bacteria (Escherichia Coli, Vibro fischeri | 2 x 4 min at 30 Hz | <40 mg microbial pellet + 140 µl BE, 10 µl Proteinase K, 2.5 µl RNaseA | MN 96 Bead Plate Type B | NucleoSpin Microbial DNA |
| Gram positive bacteria (Bacillus subtilis, Corynebacterium glutamicum) | 6-12 min at 30 Hz | <40mg microbial pellet + 140 µl BE, 10 µl Proteinase K, 2.5 µl RNaseA | MN 96 Bead Plate Type B | NucleoSpin Microbial DNA |