MN Bead Tubes Type A (DNA and nuclease free)
Microbiome samples are complex and require a very thorough lysis procedure. We recommend mechanical homogenization with ceramic bead based materials to achieve unbiased results for microbiome studies.
Soil, stool, water, air
Type A (ceramic) 0.6 - 0.8 mm ceramic beads
Compatible with the following kits
Scalable options
Solutions for small sample amounts
- 2 mL screw cap tubes
- DNA and nuclease free
- Prefilled with 0.6 - 0.8 mm ceramic beads
- compatible with common bead beating devices
- click here for more information
Solutions for larger sample amounts
- 5 mL screw cap tubes
- Prefilled with 0.6 - 0.8 mm ceramic beads
- compatible with common bead beating devices
- click here for more informaiton
HTP solution
- Pack of prefilled tube strips (8x12)
- Prefilled with 0.6 - 0.8 mm ceramic beads
- compatible with common plate disruption device
- click here for more information
Bulk solutions for different sample amounts
- bulk beads
- DNA and nuclease free
- 0.6–0.8 mm ceramic beads
- compatible with common bead beating devices
- click here for more information
Sample processing recommendations
In order to obtain optimal yields of DNA from sample material, a complete disruption of the sample material is necessary. Sample disruption efficiency depends on the parameters such as type of bead tube or bead plate, time and frequency and also the disruption device. Please find below our suggested recommendations for the MN Bead Tubes Type A in combination with different nucleic acid purification kits.
MN Bead Tube Holder in combination with a Vortex-Genie 2
Sample disruption using the MN Bead Tube Holder is a very straight forward and easy method to homogenize your sample. The MN Bead Tube Holder is available for 2 ml and 5 ml bead tubes.
| Sample type | Processing time | Buffer and volume | Recommended kit |
| Soil | Approx. 4 min full speed, RT | according to the standard protocol | NucleoMag DNA Microbiome |
| Soil | Approx. 5 min, full speed, RT | according to the standard protocol | NucleoSpin Soil |
| Soil | 2700 rpm for 5 min, RT | according to the standard protocol | NucleoBond RNA Soil |
| Stool | Approx. 5-10 min, full speed, RT | according to the standard protocol | NucleoMag DNA Microbiome |
| Stool | Approx. 10 min, full speed, RT | according to the standard protocol | NucleoSpin DNA Stool |
| Stool | Approx. 10 min, full speed, RT | according to the standard protocol | NucleoSpin RNA Stool |
| Stool | Approx. 10 min, full speed, RT | according to the standard protocol | NucleoSpin 96 DNA Stool |
| Biofilm | Approx. 5-10 min, full speed, RT | according to the standard protocol | NucleoMag DNA Microbiome |
| 25 mm filter membranes | Approx. 4 min, full speed, RT | according to the standard protocol | NucleoMag DNA/RNA Water |
| 47 mm filter membranes | Approx. 10 min, full speed, RT | according to the standard protocol | NucleoMag DNA/RNA Water |
Sample processing recommendation using a RETSCH Mixer Mill
An alternative in sample disruption of biological materials are bead beating mills. MN Bead Tubes (with the exception of MN Bead Tubes Type F) can be combined with most disruption devices. Please find below the recommendation for MN Bead Tubes Type A incombination with different purification kits and the RETSCH Mixer Mill. Click here to find our latest Application Note for MN Bead Tubes and the Retsch Mixer Mill.
MN Bead Tubes Type A, DNA and nuclease free
| Sample type | Processing time | Buffer and volume (supplementary) | Bead Tube | Recommended kit |
| Soil, stool, biofilm | 1-2 min st 30 Hz | <200 mg sample + 700 µl MI1 (Optional: 150 µl Enhancer SX, 2.5 µL RNase A) | MN Bead Tube Type A | NucleoMag DNA Microbiome |
MN 96 Bead Plate A
| Sample type | Processing time | Buffer and volume (supplementary) | Bead Tube | Recommended kit |
| Soil, stool, biofilm | 2x3 min at 30 Hz | <200 mg + 700 µl MI1 (Optional: 150 µl Enhancer SX, 2.5 µl RNase A) | MN Bead Plate Type A | NucleoMag DNA Microbiome |