Automate your nucleic acid purification with Waters and MACHEREY-NAGEL
MACHEREY-NAGEL and Waters have joined forces to optimize your automated nucleic acid extraction workflow and deliver maximal flexibility in the technology of your choice.
Enjoy the fusion of our NucleoBond Xtra Midi (EF) and NucleoSpin 96 technologies on Waters Andrew+ liquid handling robot.
Your advantages
- Maximal flexibility in technology
- Perform high-throughput of silica-membrane based NucleoSpin 96 preparations using the Extraction+ vacuum module
- Perform large-scale NucleoBond Xtra Midi preparations on the Andrew+ via gravity flow
- Reproducible performance and excellent yields
- Verified scripts and protocol information for your method safety
- Customizes scripts through a close collaboration between Waters and MN to perfectly match your specific need
- Worry-free set-up and workflows due to excellent technical support platform specific support through the experts from Waters and MN
Our support
Together with our partner Waters, we work closely together to optimize your automation experience. Our aim is to make your daily lab routine easier and free up your time. For scripts and more tailored solution for MACHEREY-NAGEL kits on the Andrew+, please contact us.
To answer all your questions regarding the automation of MACHEREY-NAGEL kits on the Andrew+, please contact our support team. Our collaboration with Waters speeds up the answering process as well.
Contact us now to elevate your research with the power to MACHEREY-NAGEL and Waters synergy.
We support you every step of the way to automate your genomic workflow.
The Andrew+
The Andrew+ offers fully automated liquid handling using electronic pipettes and labware holders called dominos. Combined with the vacuum module Extraction+, the Andrew+ is suitable for processing 96-well platers. The step-by-step protocols are available in Waters OneLab Library. In combination with NucleoBond Xtra Midi column domino, the Andrew+ is also suitable to process our NucleoBond Xtra Midi columns (Step-by step protocol in OneLab Library).
One robot – two automated technologies
NucleoBond Xtra Midi
- Automation of plasmid midi preps with anion exchange technology
- Excellent puritiy with low endotoxin levels
- Gravity-flow
NucleoSpin 96
- Automation of 96-well silica-membrane based plates
- Vacuum processed with Extraction+ module
Plasmid Purifications on the Andrew+
Medium and large-scale plasmid DNA preparations for transfection play an important role in many fields of research as well as the pharma and biotech industry. Obtaining sufficient amounts of transfection-grade plasmid DNA is more important than ever.
Anion exchange columns are successfully used to purify large amounts of highly pure plasmid DNA and remain the tool of choice for large-scale, high purity plasmid isolation. While providing significant advantages, most anion exchange columns are also slow and labor intensive.
To tackle these issues, MACHEREY‑NAGEL and Waters have combined their strengths to automate NucleoBond Xtra Midi and NucleoBond Xtra Midi EF, the fastest anion exchange column on the market with Andrew+, a highly flexible and dexterous liquid handler robot. The protocol enables completely automated purification of more than 2400 μg of transfection-grade plasmid DNA per run (6 preps with >400 µg each).
NucleoBond Xtra Plasmid Preps automated on Andrew+
Transformed (plasmid-containing) bacterial cells are lysed by an optimized set of buffers. After equilibration of the NucleoBond Xtra Column and NucleoBond Xtra Column Filter, the entire lysate is loaded by gravity flow and simultaneously cleared by the NucleoBond Xtra Column Filter. Plasmid DNA is bound to the NucleoBond Xtra Silica Resin. After efficient washing steps, the plasmid DNA is eluted and desalted by magnetic beads. Click here to see our Flyer about automated plasmid midi preps.
Additionally, MACHEREY‑NAGEL has developed a new generation of silica-membrane-based plasmid DNA purification – NucleoSpin 96 Plasmid Transfection-grade Plus – that deliver high plasmid yields and extremely low endotoxin levels. This kit combines fast, automated processing with novel endotoxin removal wash buffers, enabling convenient and time saving isolation of transfection-grade DNA (≤ 10 EU/μg with 3 wash steps, ≤ 1 EU/μg with 4 wash steps).
Application notes
Find below our application notes together with Waters. Click on the pictures and read the application note. If you have any questions please contact us.
NucleoBond Xtra Midi
- Anion exchanger for time saving transfection-grade to endotoxin-free plasmid DNA
- High yield (Typical 500 µg)
To get more detailed information on the scripts and protocols, please click the following link to Waters OneLab Library
NucleoSpin 96 kits
- Processed with the vacuum-module Extraction+
- Silica-membrane based extraction
To get more detailed information on the scripts and protocols, please click the following link to Waters OneLab Library
OneLab - Execute MACHEREY-NAGEL protocols in OneLab
Waters OneLab software offers you a complete on-screen, step by step guide through the protocol and allows to track each protocol step. Our NucleoBond, NucleoSpin 96 and NucleoSpin Protocols can be found in the library. Please find a list of protocols available in the OneLab library in the attached flyer.
