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NucleoBond® Xtra Midi / Maxi
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Transfection-grade plasmid DNA
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Blue LyseControl –
efficient alkaline lysis
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| Features |
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| The new generation of anion exchanger – ultra-fast, transfection-grade plasmid DNA with high yield |
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• Optimized column design – Midi prep in approximately 30 min, Maxi prep in 35 min
• Column filter included – high filter flow rates, parallel lysate clearing, and loading onto the column
• Improved silica material – typical yields of 400 μg (Midi) and 1 000 μg (Maxi)
• Proven anion-exchange technology – transfection-grade plasmid DNA
• NucleoBond® Xtra Plus kits contain NucleoBond® Finalizers to speed up DNA precipitation
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NucleoBond® Xtra Midi |
NucleoBond® Xtra Maxi |
| Technology |
Anion-exchange chromatography |
Anion-exchange chromatography |
| Format |
Midi gravity flow columns |
Maxi gravity flow columns |
| Lysate clarification |
Column filters |
Column filters |
| Sample material |
< 200 mL (high copy)
< 400 mL (low copy) |
< 600 mL (high copy)
< 1200 mL (low copy) |
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Vector size
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< 300 kbp |
< 300 kbp |
| Typical yield |
400 µg |
1000 µg |
| A260/A280 |
1.80–1.95 |
1.80–1.95 |
| Isopropanol precipitation |
Xtra Midi
Centrifugation
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Xtra Midi Plus
NucleoBond® Finalizer
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Xtra Maxi
Centrifugation
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Xtra Maxi Plus
NucleoBond® Finalizer Large
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| Preparation time |
70 min/prep |
30 min/prep |
75 min/prep |
35 min/prep |
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| Applications |
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• High-copy plasmids from E. coli
• Low-copy plasmids from E. coli (increased buffer volumes required, see "Ordering information") |
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Ordering information
| Product |
Preps
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Specification |
REF |
| NucleoBond® Xtra Midi kits |
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| NucleoBond® Xtra Midi |
10
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NucleoBond® Xtra Midi Columns with inserted NucleoBond® Xtra Column Filters, buffers, RNase A |
740410.10 |
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50
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as above |
740410.50 |
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100
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as above |
740410.100 |
| NucleoBond® Xtra Midi Plus |
10
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NucleoBond® Xtra Midi Columns with inserted NucleoBond® Xtra Column Filters, NucleoBond® Finalizers, buffers, RNase A
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740412.10 |
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50
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as above |
740412.50 |
| NucleoBond® Xtra Maxi kits |
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| NucleoBond® Xtra Maxi |
10
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NucleoBond® Xtra Maxi Columns with inserted NucleoBond® Xtra Column Filters, buffers, RNase A |
740414.10 |
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50
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as above |
740414.50 |
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100
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as above |
740414.100 |
| NucleoBond® Xtra Maxi Plus |
10
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NucleoBond® Xtra Maxi Columns with inserted NucleoBond® Xtra Column Filters, NucleoBond® Finalizers Large, buffers, RNase A
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740416.10 |
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50
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as above |
740416.50 |
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| Product accessories |
Pack of
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Specification |
REF |
| NucleoBond® Xtra Combi Rack |
1
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for use with NucleoBond® Xtra Midi, Maxi, BAC, AX 100, AX 500, AX 2000, and AX 10000, BAC 100 Columns |
740415 |
| NucleoBond® Smart Rack |
1
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for use with NucleoBond® Xtra Midi, Xtra Maxi,Xtra BAC, AX 20, AX 100,AX 500, AX 2000, and AX 10000, |
740413 |
| NucleoBond® Xtra Buffer Set I |
1
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for isolation of low-copy plasmids, cosmids, BACs, PACs, and P1 constructs,
only applicable with NucleoBond® Xtra kits, sufficient for 12 Xtra Maxi preps or 18 Xtra Midi preps. Set includes 150 mL buffers RES, LYS, NEU, and RNase A |
740417 |
For ordering click here
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