|
|
NucleoBond® Xtra Midi / Maxi
|
Transfection-grade plasmid DNA
|
|
Blue LyseControl –
efficient alkaline lysis
|
|
Features |
|
The new generation of anion exchanger – ultra-fast, transfection-grade plasmid DNA with high yield |

• Optimized column design – Midi prep in approximately 30 min, Maxi prep in 35 min
• Column filter included – high filter flow rates, parallel lysate clearing, and loading onto the column
• Improved silica material – typical yields of 400 μg (Midi) and 1 000 μg (Maxi)
• Proven anion-exchange technology – transfection-grade plasmid DNA
• NucleoBond® Xtra Plus kits contain NucleoBond® Finalizers to speed up DNA precipitation
|
|
|
|
|
NucleoBond® Xtra Midi |
NucleoBond® Xtra Maxi |
Technology |
Anion-exchange chromatography |
Anion-exchange chromatography |
Format |
Midi gravity flow columns |
Maxi gravity flow columns |
Lysate clarification |
Column filters |
Column filters |
Sample material |
< 200 mL (high copy)
< 400 mL (low copy) |
< 600 mL (high copy)
< 1200 mL (low copy) |
Vector size
|
< 300 kbp |
< 300 kbp |
Typical yield |
400 µg |
1000 µg |
A260/A280 |
1.80–1.95 |
1.80–1.95 |
Isopropanol precipitation |
Xtra Midi
Centrifugation
|
Xtra Midi Plus
NucleoBond® Finalizer
|
Xtra Maxi
Centrifugation
|
Xtra Maxi Plus
NucleoBond® Finalizer Large
|
Preparation time |
70 min/prep |
30 min/prep |
75 min/prep |
35 min/prep |
|
Applications |
|
• High-copy plasmids from E. coli
• Low-copy plasmids from E. coli (increased buffer volumes required, see "Ordering information") |
|
|
|
Ordering information
Product |
Preps
|
Specification |
REF |
NucleoBond® Xtra Midi kits |
|
|
|
NucleoBond® Xtra Midi |
10
|
NucleoBond® Xtra Midi Columns with inserted NucleoBond® Xtra Column Filters, buffers, RNase A |
740410.10 |
|
50
|
as above |
740410.50 |
|
100
|
as above |
740410.100 |
NucleoBond® Xtra Midi Plus |
10
|
NucleoBond® Xtra Midi Columns with inserted NucleoBond® Xtra Column Filters, NucleoBond® Finalizers, buffers, RNase A
|
740412.10 |
|
50
|
as above |
740412.50 |
NucleoBond® Xtra Maxi kits |
|
|
|
NucleoBond® Xtra Maxi |
10
|
NucleoBond® Xtra Maxi Columns with inserted NucleoBond® Xtra Column Filters, buffers, RNase A |
740414.10 |
|
50
|
as above |
740414.50 |
|
100
|
as above |
740414.100 |
NucleoBond® Xtra Maxi Plus |
10
|
NucleoBond® Xtra Maxi Columns with inserted NucleoBond® Xtra Column Filters, NucleoBond® Finalizers Large, buffers, RNase A
|
740416.10 |
|
50
|
as above |
740416.50 |
|
|
|
|
Product accessories |
Pack of
|
Specification |
REF |
NucleoBond® Xtra Combi Rack |
1
|
for use with NucleoBond® Xtra Midi, Maxi, BAC, AX 100, AX 500, AX 2000, and AX 10000, BAC 100 Columns |
740415 |
NucleoBond® Smart Rack |
1
|
for use with NucleoBond® Xtra Midi, Xtra Maxi,Xtra BAC, AX 20, AX 100,AX 500, AX 2000, and AX 10000, |
740413 |
NucleoBond® Xtra Buffer Set I |
1
|
for isolation of low-copy plasmids, cosmids, BACs, PACs, and P1 constructs,
only applicable with NucleoBond® Xtra kits, sufficient for 12 Xtra Maxi preps or 18 Xtra Midi preps. Set includes 150 mL buffers RES, LYS, NEU, and RNase A |
740417 |
For ordering click here
|
|
|
|
 |