NucleoSpin Finisher Midi for desalting and concentration of plasmid DNA
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Délai de livraison env. 5 jours
Fast concentration and desalting of plasmid DNA by centrifugation
Application | Concentration et dessalage d'éluats de plasmides |
Unité de vente | 10 Prep(s), 50 Prep(s) |
Cible | ADN plasmidique |
Certifié CE | Non, uniquement pour la recherche (RUO) |
Technologie | Précipitation et filtration |
Marque | NucleoSpin |
Format | Colonne entonnoir |
Utilisation | Centrifugation |
Utilisation automatique | Non |
Matériaux de départ | Bactérie, E. coli |
Quantité d'échantillon | Eluat d'ADN |
Taille du vecteur | < 25 kbp |
Recouvrement type | 90−100 % |
Capacité de fixation théorique | 1.5 mg |
Volume d'élution | ≥ 100 µL |
Temps de préparation | 15 min/6 preps |
Applications types | Transfection de cellules sensibles et injections de cellules |
Température de stockage | 15–25 °C |
Stabilité (à partir de la production) | 27 Moi(s) |
Substances dangereuses | Non |
Plasmid DNA - Desalting and Concentraction
Following the elution of pure plasmid DNA from the NucleoBond columns, MN provides a range of options for desalting and concentrating the plasmid. In addition to the standard isopropanol precipitation followed by centrifugation we offer the NucleoBond Finalizers for manaul desalting with a syringe as well as centrifuge-compartible NucleoSPin Finisher and vacuum compartible NucleoSnap Finisher for a fast, simple and easy prep.
![](https://www.mn-net.com/media/image/e5/a5/19/Plasmid_DNA_Desaltingoptions.jpg)
Isopropanol precipitation (traditional method, duration 1 h)
NucleoBond Finalizer and Finazlizer Large (ideal for small number of samples, duration 5 min/sample)
NucleoMag Desalting Beads (can be automated on the Andrew+ in combination with NucleoBond Xtra Midi kits)
NucleoSpin Finisher (for parallel processing of samples, with appropriate centrifuge, duration < 10 min/12 samples)
NucleoSnap Finisher Midi and Maxi (for parallel processing of samples, vacuum manifold, duration < 10 min/12 samples)
NucleoBond Finalizer
NucleoSpin and NucleoSnap Finisher
NucleoSpin Finisher are designed for quick concentration and desalting of plasmid eluates obtained by anion-exchange chromatography. Finisher replace tedious and time-consuming isopropanol precipitation. Furthermore, problems like lost DNA pellets or incomplete solubilization of hardly visible precipitates can easily be circumvented.
NucleoMag Desalting Beads
NucleoMag Desalting beads are designed for quick concentration and desalination of plasmid DNA eluates that are obtained be anion exchange chromatographic plasmid purification kits. Plasmid DNA is bound reversibly to NucleoMag Desalting beads followed by ethanolic wash steps. Finally pure plasmid DNA is eluted using a low salt buffer. NucleoMag Desalting beads can be automated on e.g the Andrew+ platform.
The NucleoBond Finalizers are designed to concentrate and desalinate plasmid DNA eluates collected using NucleoBond columns after anion-exchange chromatographic DNA purification. The sample is syringe-loaded onto a NucleoBond Finalizer membrane to avoid tedious centrifugation steps.