NucleoBond 96 Xtra EF, 96-well kit for endotoxin-free plasmid DNA, MN

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Item number: 740430.4 031010414001

Package unit 384 Preps

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2nd generation of anion exchanger for time saving endotoxin-free plasmid DNA more

2nd generation of anion exchanger for time saving endotoxin-free plasmid DNA

Application	Endotoxin-free plasmid DNA isolation
Selling unit	96 Prep(s), 384 Prep(s)
Target	Plasmid DNA
CE certified	No, research use only
Technology	Anion exchange chromatography
Brand	NucleoBond
Format	96-well plate
Handling	Gravity flow , vacuum for filtration and precipitation
Lysate clarification	NucleoBond Filter Plate
Automated use	No
Sample material	Bacteria, E. coli
Sample amount	1−5 mL
Vector size	< 25 kbp, < 300 kbp (without the NucleoBond Finalizer Plate)
Typical yield	2−4 µg (1.5 mL in 96-well plates), 10−50 µg (5 mL in glass tubes)
Theoretical binding capacity	50 µg
Typical purity A260/A280	1.8−1.95
Endotoxin level	< 0.1 EU/µg DNA
Isopropanol precipitation	Centrifugation, NucleoBond Finalizer Plate
Elution volume	100−200 µL
Preparation time	120 min/plate
Typical downstream application	Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells
Storage temperature	15–25 °C / 59–77 °F
Shelf life (from production)	39 Month(s)
Hazardous material	Yes