NucleoMag Desalting Beads zur Entsalzung und Konzentration von Plasmid DNA

263,00 $*

*ohne Steuern und Versandkosten

REF 744410.50

Packungseinheit 50 Preps

Haben Sie Fragen?

Bitte kontaktieren Sie uns.

Convenient and scalable desalting of anion exchange eluates using magnetic beads.

Anwendung	Konzentrierung und Entsalzung von Plasmid-Eluaten
Automatische Nutzung	Ja
CE-zertifiziert	Nein, nur für wissenschaftlichen Gebrauch
Elutionsvolumen	200−2000 µL (Abhängig von Plasmid DNA Konzentration and Menge)
Format	Magnetic beads
Gefahrstoffe	Nein
Gewicht Brutto (ink. Verpackung)	112.3 g
Handhabung	Manuell oder automatisiert
Lagertemperatur	15–25 °C
Präparationszeit	25 min/Präp
Probenmaterial	Bakterien, E. coli
Probenmenge	5 mL NucleoBond Xtra Midi Eluat, skalierbar von 1 - 100 mL
Technologie	Magnetische Bead-Technologie
Typische Downstream-Anwendung	Sequenzierung
Typische Rückgewinnung	≥90 %
Verpackungsabmessung	53 x 53 x 104 mm
Warenzeichen	NucleoMag
Ziel	Plasmid DNA

digitalAssets.sds.languageFieldHint

Leaflet NucleoMag Desalting Beads

Flyer Andrew Alliance

Plasmid DNA

Desalting and Concentraction

Following the elution of pure plasmid DNA from the NucleoBond columns, MN provides a range of options for desalting and concentrating the plasmid. In addition to the standard isopropanol precipitation followed by centrifugation we offer the NucleoBond Finalizers for manual desalting with a syringe as well as centrifuge-compatible NucleoSpin Finisher and vacuum compatible NucleoSnap Finisher for a fast, simple, and easy prep.

Isopropanol precipitation
(traditional method, duration 1 h)

NucleoBond Finalizer and Finalizer Large (ideal for small number of samples, duration 5 min/sample)
NucleoBond Finalizer Finalizer Large

NucleoSpin Finisher (for parallel processing of samples, with appropriate centrifuge, duration < 10 min/12 samples)
NucleoSpin Finisher

NucleoSnap Finisher Midi and Maxi (for parallel processing of samples, vacuum manifold, duration < 10 min/12 samples)
NucleoSnap Finisher Midi NucleoSnap Finisher Maxi

NucleoMag Desalting Beads (can be automated on the Andrew+ in combination with NucleoBond Xtra Midi kits)
NucleoMag Desalting Beads

NucleoBond Finalizer

The NucleoBond Finalizers are designed to concentrate and desalinate plasmid DNA eluates collected using NucleoBond columns after anion-exchange chromatographic DNA purification. The sample is syringe-loaded onto a NucleoBond Finalizer membrane to avoid tedious centrifugation steps.

NucleoSpin and NucleoSnap Finisher

NucleoSpin Finisher are designed for quick concentration and desalting of plasmid eluates obtained by anion-exchange chromatography. Finisher replace tedious and time-consuming isopropanol precipitation. Furthermore, problems like lost DNA pellets or incomplete solubilization of hardly visible precipitates can easily be circumvented.

NucleoMag Desalting Beads

NucleoMag Desalting beads are designed for quick concentration and desalination of plasmid DNA eluates that are obtained be anion exchange chromatographic plasmid purification kits. Plasmid DNA is bound reversibly to NucleoMag Desalting beads followed by ethanolic wash steps. Finally pure plasmid DNA is eluted using a low salt buffer. NucleoMag Desalting beads can be automated on e.g the Andrew+ platform.