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NucleoMag® Plant

Application notes
Application notes NucleoMag® Plant

  
     
NucleoMag® Plant

Genomic DNA from plant

Features 

Magnetic-bead based isolation of genomic DNA from plant tissue

• One-tube procedure minimizes risk of cross-contamination
• Small elution volumes 50 μL possible
• Yield does not depend on elution volume
• Easily adapted to automated use

Technology Magnetic-bead technology
Format Highly reactive superparamagnetic beads
Processing

Manual or automated

Sample material

20–50 mg plant tissue (wet weight)

Fragment size 300 bp–approx. 50 kbp
Typical yield 10–20 µg (50 mg plant tissue, wet weight)
A260/A280 1.6–1.9
Elution volume 50–200 µL
Preparation time 40–120 min/96 preps (excl. lysis)*
Binding capacity 0.4 µg/µL beads
*Depending on instrument type, setup, and configuration.
Applications
• Manual or automated isolation of DNA from plant tissue
• Typical downstream applications: PCR, Southern blotting, any kind of enzymatic reaction

 

  
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Ordering information

Product

Preps

Specification REF
NucleoMag® Plant

1 x 96

NucleoMag® C-Beads, Elution Plate U-bottom, buffers, RNase A 744400.1

4 x 96

as above 744400.4

24 x 96

as above 744400.24
Material to be supplied by the user
E.g., Rack of Tube Strips (lysis tubes)

4 sets

1 set consists of 1 rack, 12 strips with 8 tubes each, and 12 cap strips
740477
24 sets as above 740477.24
E.g., Square-well Block (separation plate)

4

96-well blocks with 2.1 mL square wells 740481

24

as above 740481.24

 

For use with KingFisher® 96 platform

 

KingFisher® 96 Accessory Kit B

1 set

Square-well Blocks, Deep-well Tip Combs and Elution Plates for 4 x 96 preparations 744951
Product accessories

Pack of

Specification REF
NucleoMag® SEP

1

magnetic separator 744900

For ordering click here

  
   
  
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