Editors
Instruction leaflet:
NUCLEOSIL®
RP columns


NUCLEOSIL® standard C8 phases    
Octyl modified silica phases for HPLC
C8 ec: medium density modification, endcapped · 9 % C · USP L7
C8: not encapped · USP L7
Features
Nonpolar phases for RP chromatography
NUCLEOSIL® C8 ec:
Endcapped, pore size 100 Å, particle size 5 µm
Separation mechanism based on hydrophobic (van der Waals) interactions, slight residual silanol interactions
Corresponding NUCLEODUR® phase: see NUCLEODUR® C8 ec
NUCLEOSIL® C8:
Not endcapped, available pore sizes 100, 120, and 300 Å,
available particle sizes 3 µm, 5 µm, 7 µm and 10 µm
Separation mechanism based on hydrophobic (van der Waals) interactions, noticeable silanol interactions
pH stability at 20 °C for both types: 2–8; carbon content depends on phase (see tables)
  For applications click here.

EC standard columns for analytical HPLC
Ordering information
Eluent in column acetonitrile – water
Length 125 mm 150 mm 250 mm   EC guard columns*
NUCLEOSIL® 100-5 C8 ec · 5 µm particles · 100 Å pores · 9 % C · endcapped    
4.6 mm ID     720165.46   721096.30
NUCLEOSIL® 100-5 C8 · 5 µm particles · 100 Å pores · 8.5 % C · not endcapped    
4 mm ID 720001.40   720013.40   721194.30
4.6 mm ID 720001.46 720990.46 720013.46   721194.30
NUCLEOSIL® 100-7 C8 · 7 µm particles · 100 Å pores · 8.5 % C · not endcapped    
4.6 mm ID     720017.46      
NUCLEOSIL® 100-10 C8 · 10 µm particles · 100 Å pores · 8.5 % C · not endcapped    
4 mm ID     720022.40      
4.6 mm ID      720022.46      
NUCLEOSIL® 120-3 C8 · 3 µm particles · 120 Å pores · 6.5 % C · not endcapped    
4 mm ID 720071.40       721093.30
4.6 mm ID 720071.46 720214.46     721093.30
NUCLEOSIL® 120-5 C8 · 5 µm particles · 120 Å pores · 6.5 % C · not endcapped    
4 mm ID 720050.40   720052.40   721095.30
4.6 mm ID 720050.46 720735.46 720052.46   721095.30
NUCLEOSIL® 300-5 C8 · 5 µm particles · 300 Å pores · ~ 3 % C · not endcapped    
4.6 mm ID     720062.46   721061.30
Columns/pack 1 1 1   3
* EC 4/3 guard column cartridges are used for EC columns of 4 and 4.6 mm ID with the Column Protection System guard column holder (REF 718966).
Custom-packed EC columns with other dimensions are available on request.

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