NUCLEOSIL® standard silica phases for HPLC

NUCLEOSIL® is a family of totally porous spherical silicas with a very pure and uniform SiO2 structure
Wide acceptance as routine packings for very different fields of chromatography
One of the first spherical silicas used in HPLC
Developed in the early seventies, it became a world-renowned HPLC packing
Still found in many analytical and preparative applications
An absolutely reliable choice in HPLC
The largest variety of modified HPLC silicas available on the market

Summary of available NUCLEOSIL® phases
Phase Modification Formula
RP phases
NUCLEOSIL® C18 medium density octadecyl, endcapped -(CH2)17-CH3
NUCLEOSIL® C18 AB crosslinked octadecyl, endcapped, high steric selectivity -(CH2)17-CH3
NUCLEOSIL® C18 HD high density octadecyl, endcapped -(CH2)17-CH3
NUCLEOSIL® C18 Nautilus hydrophilic octadecyl, embedded polar group, endcapped -(CH2)17-CH3
NUCLEOSIL® Protect I special RP phase with protective polar group, endcapped  
NUCLEOSIL® C8 ec medium density octyl, endcapped -(CH2)7-CH3
NUCLEOSIL® C8 standard octyl, not endcapped -(CH2)7-CH3
NUCLEOSIL® C8 HD high density octyl, endcapped -(CH2)7-CH3
NUCLEOSIL® C4 medium density butyl, endcapped -(CH2)3-CH3
NUCLEOSIL® C2 dimethyl -(CH3)2
NUCLEOSIL® C6H5 standard phenyl, not endcapped -(CH2)3-C6H5
Polar phases
NUCLEOSIL® CN cyanopropyl (nitrile) -(CH2)3-CN
NUCLEOSIL® OH diol (dihydroxypropyl) -(CH2)3-O-CH2-CH(OH)-CH2OH
NUCLEOSIL® NH2 aminopropyl -(CH2)3-NH2
NUCLEOSIL® N(CH3)2 dimethylamino -(CH2)3-N(CH3)2
NUCLEOSIL® SA benzenesulfonic acid, strongly acidic cation exchanger (SCX) -(CH2)3-C6H4-SO3Na
NUCLEOSIL® SB quaternary ammonium groups, strongly basic anion exchanger (SAX) -(CH2)3-C6H4-CH2-N+(CH3)3Cl
NUCLEOSIL® SiOH unmodified (SiO2)n

Technical characteristics
Spherical silica, pH stability 2–8
Available pore sizes 50, 100, 120, 300, 500, 1000 and 4000 Å
Available particle sizes 3 µm, 5 µm, 7 µm and 10 µm
  Physical properties of unmodified NUCLEOSIL® materials
  Phase Pore size Pore volume Surface (BET) Density Pressure stability*
  NUCLEOSIL 50 50 Å 0.8 mL/g 420 m2/g 0.45 g/mL 500 bar
  NUCLEOSIL 100 100 Å 1 mL/g 350 m2/g 0.36 g/mL 500 bar
  NUCLEOSIL 120 120 Å 0.65 mL/g 200 m2/g 0.55 g/mL 500 bar
  NUCLEOSIL 300 300 Å 0.8 mL/g 100 m2/g 0.45 g/mL 400 bar
  NUCLEOSIL 500 500 Å 0.8 mL/g 35 m2/g 0.45 g/mL 400 bar
  NUCLEOSIL 1000 1000 Å 0.8 mL/g 25 m2/g 0.45 g/mL 300 bar
  NUCLEOSIL 4000 4000 Å 0.7 mL/g 10 m2/g 0.48 g/mL 300 bar
  * Maximum packing pressure; for maximum working pressure of packed columns please refer to the instruction leaflets.

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