User manual NucleoSpin® Plant II

NucleoSpin® Plant II

Genomic DNA from plant and fungi

Two lysis buffers –
solution for any plant material
The second generation for rapid isolation of genomic DNA from plant samples
with higher yield and quality

• Two alternative lysis buffers included for optimal processing of various samples
   and highest flexibility:
   Lysis Buffer PL1, based on CTAB lysis method
   Lysis Buffer PL2, based on SDS lysis method
• NucleoSpin® Filters included for clarification of lysate
• Improved buffer compositions – higher yield and purity
• Optimized silica membrane – improved DNA binding
• RNase A included
Technology Silica-membrane technology
Format Mini spin columns
Sample material

< 100 mg plant tissue (wet weight), < 20 mg plant tissue (dry weight)

Lysate clarification NucleoSpin® Filters
Fragment size 50 bp–approx. 50 kbp
Typical yield 1–30 µg (100 mg plant tissue, wet weight)
A260/A280 1.8–1.9
Elution volume 50–100 µL
Preparation time 30 min/prep
Binding capacity 50 µg
• DNA from plant cells and tissue
• DNA from fungi
• Typical downstream applications: PCR, Southern blotting, enzymatic reactions


Ordering information



Specification REF
NucleoSpin® Plant II


NucleoSpin® Plant II Columns, NucleoSpin Filters, Collection Tubes (2 mL), buffers, RNase A 740770.10


as above 740770.50


as above 740770.250

For ordering click here

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