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Protino® 96 Ni-IDA

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Protino® Ni-TED/IDA

  
     

Protino® 96 Ni-IDA

Purification of His-tag proteins

Features 
Protino® Ni-IDA, best choice for higher yields of His-tag proteins
• Chelating group IDA (iminodiacetic acid), three protein binding sites
• High protein yield/recovery even from diluted samples
• High protein concentration
• High binding specificity, less unspecific binding of contaminating proteins
   compared to other common IMAC matrices
• Dry resin – storage at room temperature 

• Ready-to-use prepacked 96-well plates filled with dry Protino® Ni-IDA Resin 
• Buffers included

Technology IMAC (immobilized metal ion affinity chromatography)
Chelating group IDA (iminodiacetic acid)
Format 96-well gravity-flow plates
Matrix

Macroporous silica

Physical form

Dry matrix, precharged with Ni2+

Amount of resin per well

50 mg
Bed volume 100 µL

Binding capacity*

1 mg/well

* refers to 6xHis-GFPuv

Applications
• Purification of polyhistidine-tagged proteins
• 96-well format gravity flow chromatography
  
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Ordering information

Product

Preps

Specification REF
Protino® 96 Ni-IDA

1 x 96

Protino® 96 Ni-IDA Plates, buffers 745300.1

4 x 96

as above 745300.4

For ordering click here

  
   
  
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