User manual
Protino® Ni-IDA Resin

Protino® Columns

Receiver Plates

Product flyer
Protein Purification Guide

FAQs Protino®

Product selection / Reagent compatibility
Protino® Ni-TED / IDA


Protino® Ni-IDA Resin

Purification of His-tag proteins

Protino® Ni-IDA, best choice for higher yields of His-tag proteins

• Chelating group IDA (iminodiacetic acid), three protein binding sites
   (also see Technologies)
• High protein yield / recovery even from diluted samples
• High protein concentration
• High binding specificity, less unspecific binding of contaminating proteins
   compared to other common IMAC matrices
• Dry resin – storage at room temperature

Protino® Ni-IDA Resin
• Dry bulk material
• Rigid support silica matrix, compatible with high pressure and high flow rates,
   does not shrink or swell – highest flexibility
• Batch procedure, gravity-flow column chromatography, FPLC™ applications
• Empty Protino® columns available for use with Protino® Ni-IDA Resin
• Empty 96-well Receiver Plates available for use with Protino® Ni-IDA Resin


IMAC, immobilized metal ion affinity chromatography

Chelating ligand

IDA (iminodiacetic acid)


Bulk matrix


Macroporous silica

Physical form

Dry matrix, precharged with Ni2+

Binding capacity*

20 mg/g resin
10 mg/mL bed volume

Max. pressure

 145 psi (10 bar)

* Binding capacity will vary for each polyhistidin-tagged protein
• Purification of polyhistidine-tagged proteins
• Batch binding, gravity-flow column chromatography, MPLC / FPLC™ applications


Customer testimonial

"After frustrating attempts with other resins, I was surprised at how easy it was to isolate my protein from inclusion bodies using the Protino® Ni-IDA Column and protocol from MACHEREY-NAGEL. In one day, I obtained a significant amount of nearly pure protein. I will use MACHEREY-NAGEL’s resins for all my future polyhistidine-tagged, recombinant protein purifications. " 

N. J. Davis (PhD), University of California San Francisco, Department of Medicine, Division of Infectious Disease, 513 Parnassus Ave., San Francisco, CA 94143, USA.


Ordering information



Specification REF
Protino® Ni-IDA Resin

5 g

dry silica bulk resin 745210.5

30 g

as above 745210.30

120 g

as above 745210.120

600 g

as above 745210.600

Product accessories

Pack of

Specification REF
Protino® Columns 14 mL


empty columns (gravity flow) for use with e.g. Protino® Ni-TED / IDA Resin 745250.10
Protino® Columns 35 mL


empty columns (gravity flow) for use with e.g. Protino® Ni-TED / IDA Resin 745255.10
Receiver Plate 50 µm hydrophilized


96-well plates with an inserted hydrophilized filter frit of 50 µm pore size, suitable for gravity flow, centrifugation, and vacuum 740689.4

For ordering click here

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