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Protino® Ni-IDA Packed Columns

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Protein Purification Guide

FAQs
FAQs Protino®

Product selection / Reagent compatibility
Protino® Ni-TED / IDA

  
     

Protino® Ni-IDA Packed Columns

Purification of His-tag proteins

Features
Protino® Ni-IDA, best choice for higher yields of His-tag proteins
• Chelating group IDA (iminodiacetic acid), three protein binding sites 
   (also see Technologies)
• High protein yield / recovery even from diluted samples
• High protein concentration
• High binding specificity, less unspecific binding of contaminating proteins
   compared to other common IMAC matrices
• Dry resin – storage at room temperature

Protino® Ni-IDA Packed Columns
• Ready-to-use prepacked columns filled with dry Protino® Ni-IDA Resin
• Buffers included

Protino® Ni-IDA
150 Packed Columns

Protino® Ni-IDA
1000 Packed Columns

Protino® Ni-IDA
2000 Packed Columns

Technology IMAC
(immobilized metal ion affinity chromatography)
IMAC
(immobilized metal ion affinity chromatography)
IMAC
(immobilized metal ion affinity chromatography)
Chelating ligand IDA (iminodiacetic acid) IDA (iminodiacetic acid) IDA (iminodiacetic acid)
Format Mini
gravity flow columns
Midi
gravity flow columns
Maxi
gravity flow columns
Matrix Macroporous silica Macroporous silica Macroporous silica
Physical form Dry matrix,
precharged with Ni2+
Dry matrix,
precharged with Ni2+
Dry matrix,
precharged with Ni2+

Amount of resin
per column

 40 mg 250 mg  500 mg 
Bed volume  80 µL 500 µL 1000 µL
Binding capacity* 800 µg 5 mg  10 mg 
* Binding capacity will vary for each polyhistidin-tagged protein

Applications

• Purification of polyhistidine-tagged proteins
  
Maximize    

     

Customer testimonial

"After frustrating attempts with other resins, I was surprised at how easy it was to isolate my protein from inclusion bodies using the Protino® Ni-IDA Column and protocol from MACHEREY-NAGEL. In one day, I obtained a significant amount of nearly pure protein. I will use MACHEREY-NAGEL’s resins for all my future polyhistidine-tagged, recombinant protein purifications. " 

N. J. Davis (PhD), University of California San Francisco, Department of Medicine, Division of Infectious Disease, 513 Parnassus Ave., San Francisco, CA 94143, USA.

  
     

Ordering information

Product

Preps

Specification REF
Protino® Ni-IDA 150 Packed Columns

10

Protino® Ni-IDA 150 Packed Columns, buffers 745150.10

50

as above 745150.50
Protino® Ni-IDA 1000 Packed Columns

5

Protino® Ni-IDA 1000 Packed Columns, buffers 745160.5

50

as above 745160.50
Protino® Ni-IDA 2000 Packed Columns

5

Protino® Ni-IDA 2000 Packed Columns, buffers 745170.5

25

as above 745170.25

For ordering click here

  
   
  
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