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NucleoSpin® RNA/DNA Buffer Set

RNA and DNA isolation

Features
Parallel isolation of RNA and DNA from undivided samples in one working procedure
• To be used in combination with NucleoSpin® RNA, NucleoSpin® RNA XS, NucleoSpin® miRNA,
   NucleoSpin® RNA Blood, NucleoSpin® RNA Plant, NucleoSpin® RNA/Protein kits
• No need to split samples (e. g., precious, indivisible samples like biopsy material)
• High quality DNA and RNA from one sample, perfect for PCR, RT-PCR, real-time PCR
• Fast procedure
Format Buffer set for elution of DNA in combination with RNA preparations,
to be used in combination with NucleoSpin® RNA kits
Sample material See NucleoSpin® RNA, NucleoSpin® RNA XS, NucleoSpin® miRNA,
NucleoSpin® RNA Blood, NucleoSpin® RNA Plant, NucleoSpin® RNA/Protein
DNA size

< 30 kbp

Typical DNA yield 5 µg (106 HeLa cells)
16 µg (30 mg pig liver)
5 µg (100 mg maize leaf)
A260/A280 1.7–2.0
DNA elution volume 100 µL
RNA yield and purity Identical to NucleoSpin® RNA, NucleoSpin® RNA XS, NucleoSpin® miRNA,
NucleoSpin® RNA Blood, NucleoSpin® RNA Plant, NucleoSpin® RNA/Protein

Applications*

• For biopsy material: RNA for expression analysis; DNA for mutation analysis (e.g., of cancer genes)
• For ticks (Ixodes ricinus): RNA for analysis of RNA viruses (e.g., TBE-V, Tick-borne encephalitis virus,
   infectious agent of meningoencephalitis); DNA for analysis of germs (e.g., Borrelia burgdorferi, infective
   agent of the Lyme disease)
• For transgenic plants or animals: RNA for expression analysis of the transformed gene and/or
   other genes;
   DNA for analysis of integration site, integration number and sequence confirmation of the transformed
   gene
• For transfected cultured cells: RNA for expression analysis of the transgene or other genes of interest;
   DNA for analysis of methylation status of the transgene or other genes of interest

* Kits to be used for research purposes only.
  
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Ordering information

Product

Preps

Specification REF
NucleoSpin® RNA/DNA Buffer Set

100

Buffers
sufficient for 100 DNA isolations in combination with NucleoSpin® RNA, NucleoSpin® RNA XS, NucleoSpin® RNA, NucleoSpin® miRNA, NucleoSpin® RNA Blood, NucleoSpin® RNA Plant, or NucleoSpin® RNA/Protein kits
740944

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