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| NucleoSpin® RNA Clean-up |
RNA clean-up
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| Features |
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| Simple, fast, and convenient clean-up of RNA |
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• Complete removal of RT-PCR inhibitors
• Time-saving procedure based on NucleoSpin® RNA, without DNase digestions and
homogenization steps
• RNA clean-up from pre-purified RNA (phenol/chloroform), enzymatic reactions
(e.g. amplification reactions, labeling reactions)
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| Technology |
Silica-membrane technology |
| Format |
Mini spin columns |
| Sample material |
≤ 200 µL phenol/chloroform extract, reaction mixture, or < 105 cells
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| Fragment size |
> 200 nt |
| Typical recovery |
85–95% |
| A260/A280 |
1.9–2.1 |
| Typical RIN (RNA integrity number) |
Depending on sample quality (no significant loss of RIN detected) |
| Elution volume |
40–120 µL |
| Preparation time |
20 min/6 preps |
| Binding capacity |
200 µg |
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Applications*
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RNA clean-up of:
• Pre-purified RNA (e.g., Trizol®)
• Reaction mixtures
• Amino-allyl-mRNA
• Biotinylated RNA
• RNA isolation from up to 105 cultured cells (whenever co-purification of some genomic DNA is acceptable,
kit does not contain rDNase)
• Typical downstream applications: enzymatic labeling reactions, RT-PCR, DNA/RNA-based chip
hybridizations |
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| * Kits to be used for research purposes only |
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Ordering information
| Product |
Preps
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Specification |
REF |
| NucleoSpin® RNA Clean-up |
10
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NucleoSpin® RNA Clean-up Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 mL), buffers |
740948.10 |
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50
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as above |
740948.50 |
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250
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as above |
740948.250 |
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| Product accessories |
Pack of
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Specification |
REF |
rDNase Set
click for detailed information |
1
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recombinant DNase and Reaction Buffer for rDNase, sufficient for 50 Mini preps of total RNA |
740963 |
For ordering click here
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