User manual
NucleoSpin® Gel and PCR Clean-up

Supplementary protocol
PCR Clean‑up using a vacuum manifold

Product flyer
DNA and RNA Clean-up Guide

NucleoSpin® Gel and PCR Clean-up

PCR clean-up / Gel extraction

Binding Buffer NTI –colored pH indicator

PCR clean-up and gel extraction - the two-in-one kit with optimized recovery and elution volume

• Two applications in one kit – one buffer with optimal performance for both applications
• High recoveries for fragments down to 50 bp
• Minimized elution volume of 15 μL – highly concentrated DNA
• Binding buffer with pH indicator
• Separate buffers for single stranded DNA or SDS containing samples available
• Suitable for all gel buffer systems (e.g., TAE, TBE)

Also available for QIAcube® (please see "Ordering information" and contact Technical Service
techbio@mn-net.com for more information).

Technology Silica-membrane technology
Format Mini spin columns
Sample material

< 400 µL PCR reaction mixture
< 400 mg TAE / TBE agarose gel

Fragment size 50 bp–approx. 20 kbp
Typical recovery 70–95%
A260/A280 1.8–1.9
Elution volume 15–30 µL
Preparation time 10 min/6 preps
Binding capacity 25 µg

• Purification of PCR products
• Extraction of DNA from agarose gels
• DNA extraction from polyacrylamide gels
• Clean-up of single stranded DNA and RNA extraction from agarose gels (Binding Buffer NTC required, not included in the kit, see “Ordering information”)
• Clean-up of SDS-containing samples (Binding Buffer NTB required, not included in the
   kit, see “Ordering information”)
• Typical downstream applications: cloning, sequencing, PCR, restriction analysis

• NucleoSpin® Gel and PCR Clean-up ensures complete removal of contaminations such as:
- nucleotides, primers
- enzymes
- mineral oil
- PCR additives (e.g., salts, betaine, DMSO)
- detergents (e.g., Tween 20, Triton X-100)
- dyes (e.g., ethidiumbromide, crystal violet, Stain G, Midori Green, Roti®-Safe GelStain, DNA SafeStain)


Ordering information



Specification REF
NucleoSpin® Gel and PCR Clean-up


NucleoSpin® Gel and PCR Clean-up Columns,
Collection Tubes (2 mL), buffers


as above 740609.50


as above 740609.250


Kit for use on QIAcube®*
NucleoSpin® Gel and PCR Clean-up Columns, buffers
Product accessories

Pack of

Specification REF
Buffer NTB

150 mL

for clean-up of SDS-containing samples 740595.150
Buffer NTC

100 mL

for clean-up of single stranded DNA  740654.100

* NucleoSpin® kits on QIAcube®: The usage of NucleoSpin® kits on the QIAcube® is at your own liability.
MACHEREY-NAGEL is not responsible for loss of warranty claims or other consequences.

For ordering click here

Copyright 2019 by MACHEREY-NAGEL GmbH & Co. KG   Imprint / Impressum Privacy StatementTerms Of Use