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Protino® Ni-TED Packed Columns

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Protein Purification Guide

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Protino® Ni-TED / IDA

  
     

Protino® Ni-TED Packed Columns

Purification of His-tag proteins

Features 
Protino® Ni-TED, the purest alternative for His-tag protein purification
• Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein
   binding site provides highest selectivity and purity (also see
Technologies)
• High stability against reducing / chelating agents
• Low metal leaching
• High protein purity
• High binding specificity, less unspecific binding of contaminating proteins
   compared to other common IMAC matrices
• Dry resin – storage at room temperature

Protino® Ni-TED Packed Columns
• Ready-to-use prepacked columns filled with dry Protino® Ni-TED Resin
• Buffers included


Protino® Ni-TED
150 Packed Columns
Protino® Ni-TED
1000 Packed Columns
Protino® Ni-TED
2000 Packed Columns
Technology IMAC
(immobilized metal ion affinity chromatography)

IMAC
(immobilized metal ion affinity chromatography)

IMAC
(immobilized metal ion affinity chromatography)
Chelating ligand TED (tris-carboxymethyl ethylene diamine) TED (tris-carboxymethyl ethylene diamine) TED (tris-carboxymethyl ethylene diamine)
Format Mini
gravity flow columns
Midi
gravity flow columns
Maxi
gravity flow columns
Matrix Macroporous silica Macroporous silica Macroporous silica
Physical form Dry matrix,
precharged with Ni2+
Dry matrix,
precharged with Ni2+
Dry matrix,
precharged with Ni2+

Amount of resin
per column

 40 mg 250 mg  500 mg 
Bed volume  80 µL 500 µL 1000 µL
Binding capacity* 400 µg 2.5 mg  5 mg 
* Binding capacity will vary for each polyhistidin-tagged protein

Applications

• Purification of polyhistidine-tagged proteins
  
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Ordering information

Product

Preps

Specification REF
Protino® Ni-TED 150 Packed Columns

10

Protino® Ni-TED 150 Packed Columns, buffers 745100.10

50

as above 745100.50
Protino® Ni-TED 1000 Packed Columns

5

Protino® Ni-TED 1000 Packed Columns, buffers 745110.5

50

as above 745110.50
Protino® Ni-TED 2000 Packed Columns

5

Protino® Ni-TED 2000 Packed Columns, buffers 745120.5

25

as above 745120.25

For ordering click here

  
   
  
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