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NucleoSpin®
Plasmid EasyPure


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Plasmid purification guide

  
     
NucleoSpin® Plasmid EasyPure

Plasmid Mini preps

Features

For ultra-fast small scale preparation of up to 35 µg of high-purity plasmid DNA

• LyseControl for visualization of completed alkaline lysis
• Liquid RNase A – easy handling without dissolving
• One combined washing and drying step for an ultra-fast procedure – 6 Mini preps in 14 min

Technology Silica-membrane technology
Format Mini spin columns
Sample material 2–10 mL E. coli culture
Vector size < 15 kbp
Typical yield 15—30 µg
A260/A280 1.80–1.85
Elution volume 50 µL
Preparation time 14 min/6 preps
Binding capacity 35 µg
Applications

• High-copy plasmids from E. coli
• Low-copy plasmids (increased buffer volumes required, see “Ordering information”)
• Plasmid DNA from Gram-positive bacteria
• Typical downstream applications: cloning, sequencing, PCR, transformation, restriction analysis

  
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Ordering information

Product

Preps

Specification REF
NucleoSpin® Plasmid EasyPure

10

NucleoSpin Plasmid® EasyPure Columns, Collection Tubes (2 mL), buffers, Liquid RNase A 740727.10

50

as above 740727.50

250

as above 740727.250
Product accessories

Pack of

Specification REF
NucleoSpin® Plasmid Buffer Set

1

for isolation of low-copy plasmids,
75 mL of buffers A1 and A2,
100 mL of buffers A3, RNase A;
sufficient for 300 plasmid Mini preps
740953
Liquid RNase A

250 mg

740397

For ordering click here

  
     
  
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