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NucleoBond®
BAC 100

  
     
NucleoBond® BAC 100

Transfection-grade plasmid DNA

Features 
The anion exchanger for purification of P1, BACs, PACs, and other large constructs

 

• Suitable for constructs up to 300 kbp
• Optimized lysis buffer volumes for large bacterial cultures
• NucleoBond® Folded Filters included for gentle lysate clarification without shearing of large
   constructs
Technology Anion-exchange chromatography
Format Gravity-flow columns
Lysate clarification Folded filters
Sample material 100–500 mL E.coli culture
Vector size < 300 kbp
Typical yield 10–100 µg
A260/A280 1.80–1.95
Preparation time 120 min/2–4 preps
Binding capacity 100 µg
Applications
• Purification of P1, BACs, PACs, and other large constructs from E. coli
• Typical downstream applications: cloning, PCR, restriction analysis, sequencing, and transfection
  
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Ordering information

Product

Preps

Specification REF
NucleoBond® BAC 100

10

NucleoBond® BAC 100 Columns, buffers,
RNase A, NucleoBond® Folded Filters
740579
Product accessories

Pack of

Specification REF
NucleoBond® Rack Large

1

for use with NucleoBond® AX 100, 500, 2000,
10000, BAC 100, and Xtra Midi Columns

740563
NucleoBond® Buffer Set I

1

for isolation of low-copy plasmids, cosmids, BACs, PACs, and P1 constructs,
complete buffer set and RNase A to process 5 NucleoBond® BAC 100 Columns
740601

For ordering click here

  
   
  
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