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NucleoBond® PC EF

  
     
NucleoBond® PC EF

Endotoxin-free plasmid DNA

Features 
The classic anion exchanger for purification of endotoxin-free plasmid DNA

• Plasmid DNA with less than 0.1 EU/μg for transfection of highly sensitive cells
• Patented endotoxin removal by additional washing step – no extra ice incubation necessary
• Fast and easy clarification of lysates by NucleoBond® Folded Filters, no centrifugation required,
   no shearing forces
• NucleoBond® Finalizers available as separate tool to avoid time consuming centrifugation for
   plasmid precipitation
• Proven anion-exchange technology – transfection-grade plasmid DNA
• Separate binding columns of NucleoBond® PC kits available as NucleoBond® AX columns

NucleoBond®
PC 500 EF
NucleoBond®
PC 2000 EF
NucleoBond®
PC 10000 EF
NucleoBond®
PC Prep 100
Technology

Anion-exchange chromatography

Format

Maxi

Mega

Giga

Preparative
Scale

Gravity-flow
columns

Gravity-flow
columns

Gravity-flow
columns

Compatible with
e.g., HPLC, FPLC

Lysate clarification

Folded filters

Bottle top
filters

Bottle top
filters

Folded filters,
sieving fabric

Sample material

30–150 mL

150–500 mL

500–2000 mL

5–20 L

Vector size

< 300 kbp

< 300 kbp

< 300 kbp

< 300 kbp

Typical yield

100–500 µg

500–2000 µg

2000–10000 µg

80–100 mg

A260/A280

1.80–1.95

1.80–1.95

1.80–1.95

1.80–1.95

Endotoxin level

< 0.1 EU/µg

< 0.1 EU/µg

< 0.1 EU/µg

< 0.1 EU/µg

Preparation time

100 min/2 preps

150 min/2 preps

180 min/2 preps

20 h/prep

Binding capacity

500 µg

2000 µg

10000 µg

100 mg

Applications

• High-copy plasmids from E. coli

  
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Ordering information

Product

Preps

Specification REF
NucleoBond® PC 500 EF

10

NucleoBond® AX 500 Columns, buffers, RNase A, NucleoBond® Folded Filters XL 740550
NucleoBond® PC 2000 EF

5

NucleoBond® AX 2000 Columns, buffers, RNase A, NucleoBond® Bottle Top Filters 740549
NucleoBond® PC 10000 EF

5

NucleoBond® AX 10000 Columns, buffers, RNase A, NucleoBond® Bottle Top Filters 740548
NucleoBond® PC Prep 100

1

1 NucleoBond® AX Prep 100 Column,
3 NucleoBond® AX 100 Columns, buffers, RNase A, Sieving Fabric, NucleoBond® Folded Filters
740594




Product accessories

Pack of

Specification REF
NucleoBond® Large Rack

1

for use with NucleoBond® AX 100, 500, 2000, 10000, BAC 100, and Xtra Midi Columns 740563

For ordering click here

  
   
  
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