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NucleoBond® PC

Supplementary protocol
NucleoBond® PC 10000 – low-copy plasmid purification

  
     
NucleoBond® PC

Transfection-grade plasmid DNA

Features 
The classic anion exchanger for purification of transfection-grade plasmid DNA

• Fast and easy clarification of lysates with NucleoBond® Folded Filters, no centrifugation
   required, no shearing forces
• NucleoBond® Finalizers available as separate tool to avoid time consuming centrifugation for
   plasmid precipitation
• Proven anion-exchange technology – transfection-grade plasmid DNA
• Separate binding columns of NucleoBond® PC kits available as NucleoBond® AX columns


NucleoBond®
PC 20

NucleoBond®
PC 100

NucleoBond®
PC 500

NucleoBond®
PC 2 000

NucleoBond®
PC 10 000

Technology

Anion-exchange chromatography

Format

Mini

Midi

Maxi

Mega

Giga

Gravity-flow columns

Lysate clarification

Centrifugation

Folded filters

Folded filters

Folded filters

Folded filters

Sample material 1–5 mL (HC*)
3–10 mL (LC**)
5–30 mL (HC*)
10–100 mL (LC**)
30–150 mL (HC*)
100–500 mL (LC**)
150–500 mL
(HC*)
500–2000 mL
(HC*)

Vector size

< 300 kbp

< 300 kbp

< 300 kbp

< 300 kbp

< 300 kbp

Typical yield

3–20 µg

20–100 µg

400–500 µg

500–
2000 µg

2000–
10000 µg

A260/280

1.80–1.95

1.80–1.95

1.80–1.95

1.80–1.95

1.80–1.95

Preparation time 60 min/
4–6 preps
65 min/
4–6 preps
80–90 min/
4 preps
90–120 min/
4–6 preps
120–150 min/
2 preps
Binding capacity

20 µg

100 µg

500 µg

2000 µg

10000 µg

* High-copy plasmids
** Low-copy plasmids

Applications
• High-copy plasmids from E. coli
• Low-copy plasmids from E. coli (increased buffer volumes required, see "Ordering information")
  
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Ordering information

Product

Preps

Specification REF
NucleoBond® PC kits for purification of plasmids and cosmids
NucleoBond® PC 20

20

NucleoBond® AX 20 Columns, buffers, RNase A 740571

100

as above 740571.100
NucleoBond® PC 100

20

NucleoBond® AX 100 Columns, buffers, RNase A, NucleoBond® Folded Filters 740573

100

as above

740573.100
NucleoBond® PC 500

10

NucleoBond® AX 500 Columns, buffers, RNase A, NucleoBond® Folded Filters XL 740574

25

as above 740574.25

50

as above 740574.50

100

as above 740574.100
NucleoBond® PC 2000

5

NucleoBond® AX 2000 Columns, buffers, RNase A, NucleoBond® Folded Filters XL 740576
NucleoBond® PC 10000

5

NucleoBond® AX 10000 Columns, buffers, RNase A, NucleoBond® Folded Filters (Type 1, Type 2) 740593
NucleoBond® AX columns
NucleoBond® AX 20

20

NucleoBond® AX 20 Columns 740511
NucleoBond® AX 100

20

NucleoBond® AX 100 Columns 740521

100

as above 740521.100
NucleoBond® AX 500

10

NucleoBond® AX 500 Columns

740531

50

as above 740531.50
NucleoBond® AX 2000

10

NucleoBond® AX 2000 Columns

740525
NucleoBond® AX 10000

5

NucleoBond® AX 10000 Columns

740534
Product accessories

Pack of

Specification REF
NucleoBond® accessories
NucleoBond® Small Rack

1

for use with NucleoBond® AX 20 Columns 740562
NucleoBond® Large Rack

1

for use with NucleoBond® AX 100, 500, 2000, 10000, BAC 100, and Xtra Midi Columns 740563
NucleoBond® Smart Rack

1

for use with NucleoBond® Xtra Midi, Xtra Maxi, Xtra BAC, AX 20, 100, 500, 2000, 10000 Columns 740413
NucleoBond® buffers
NucleoBond® Buffer Set I

1

for isolation of low-copy plasmids, cosmids, BACs, PACs, and P1 constructs,
only applicable with NucleoBond® PC kits, complete buffer set and RNase A to process 35 AX 20, 20 AX 100, 10 AX 500, or 2 AX 2000 Columns
740601

For ordering click here

  
     
  
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